Migration of keratinocytes through tunnels of digested fibrin

Détails

ID Serval
serval:BIB_29AAB43D5ACF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Migration of keratinocytes through tunnels of digested fibrin
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Ronfard  V., Barrandon  Y.
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
04/2001
Volume
98
Numéro
8
Pages
4504-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr 10
Résumé
We report here a hitherto undescribed form of cell migration. When a suspension of human keratinocytes is plated on a fibrin matrix, single cells invade the matrix and progress through it as rounded cells by dissolving the fibrin and thereby creating tunnels. These tunnels are cylindrical or helical, the latter being the result of constant change in the path of cellular advance around the helical axis. Helical tunnel formation is strongly promoted by epidermal growth factor. The rate of migration of the cell through the track of a helical tunnel (up to 2.1 mm per day) is about 7-fold greater than through a cylindrical tunnel. Pericellular fibrinolysis leading to tunnel formation depends on the presence of plasminogen in the medium and its conversion to plasmin by a cellular activator. Formation of tunnels requires that plasminogen activator be localized on the advancing surface of the keratinocyte; we propose that the tunnel is cylindrical when the site of release of plasmin is located at a fixed point on the cell surface and helical when the site of release precesses.
Mots-clé
*Cell Movement Cells, Cultured Epidermal Growth Factor/pharmacology Fibrin/*metabolism Fibrinolysis Humans Infant, Newborn Keratinocytes/*cytology/drug effects
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 9:41
Dernière modification de la notice
20/08/2019 14:09
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