Structural and molecular characterization of paraventricular thalamic glucokinase-expressing neuronal circuits in the mouse.

Détails

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Etat: Public
Version: de l'auteur⸱e
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_299EE8542FB3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Structural and molecular characterization of paraventricular thalamic glucokinase-expressing neuronal circuits in the mouse.
Périodique
The Journal of comparative neurology
Auteur⸱e⸱s
Gaspari S., Quenneville S., Rodriguez Sanchez-Archidona A., Thorens B., Croizier S.
ISSN
1096-9861 (Electronic)
ISSN-L
0021-9967
Statut éditorial
Publié
Date de publication
08/2022
Peer-reviewed
Oui
Volume
530
Numéro
11
Pages
1773-1949
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The thalamic paraventricular nucleus (PVT) is a structure highly interconnected with several nuclei ranging from forebrain to hypothalamus and brainstem. Numerous rodent studies have examined afferent and efferent connections of the PVT and their contribution to behavior, revealing its important role in the integration of arousal cues. However, the majority of these studies used a region-oriented approach, without considering the neuronal subtype diversity of the nucleus. In the present study, we provide the anatomical and transcriptomic characterization of a subpopulation of PVT neurons molecularly defined by the expression of glucokinase (Gck). Combining a genetically modified mouse model with viral tracing approaches, we mapped both the anterograde and the retrograde projections of Gck-positive neurons of the anterior PVT (Gck <sup>aPVT</sup> ). Our results demonstrated that Gck <sup>aPVT</sup> neurons innervate several nuclei throughout the brain axis. The strongest connections are with forebrain areas associated with reward and stress and with hypothalamic structures involved in energy balance and feeding regulation. Furthermore, transcriptomic analysis of the Gck-expressing neurons revealed that they are enriched in receptors for hypothalamic-derived neuropeptides, adhesion molecules, and obesity and diabetes susceptibility transcription factors. Using retrograde labeling combined with immunohistochemistry and in situ hybridization, we identify that Gck <sup>aPVT</sup> neurons receive direct inputs from well-defined hypothalamic populations, including arginine-vasopressin-, melanin-concentrating hormone-, orexin-, and proopiomelanocortin-expressing neurons. This detailed anatomical and transcriptomic characterization of Gck <sup>aPVT</sup> neurons provides a basis for functional studies of the integration of homeostatic and hedonic aspects of energy homeostasis, and for deciphering the potential role of these neurons in obesity and diabetes development.
Mots-clé
Animals, Glucokinase/genetics, Glucokinase/metabolism, Mice, Midline Thalamic Nuclei/metabolism, Neurons/metabolism, Obesity/metabolism, Paraventricular Hypothalamic Nucleus/metabolism, Thalamus/metabolism, TRAP, anterograde and retrograde tract tracing, glucokinase-expressing neurons, hypothalamic nuclei, neurohormones, neuropeptides, neurotransmitters, paraventricular nucleus of the thalamus
Pubmed
Web of science
Open Access
Oui
Création de la notice
31/03/2022 21:18
Dernière modification de la notice
25/01/2024 8:32
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