Cadmium uptake and induction of metallothionein synthesis in a renal epithelial cell line (LLC-PK1).

Détails

ID Serval
serval:BIB_299D68497510
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cadmium uptake and induction of metallothionein synthesis in a renal epithelial cell line (LLC-PK1).
Périodique
Archives of Toxicology
Auteur(s)
Felley-Bosco E., Diezi J.
ISSN
0340-5761 (Print)
ISSN-L
0340-5761
Statut éditorial
Publié
Date de publication
1991
Peer-reviewed
Oui
Volume
65
Numéro
2
Pages
160-163
Langue
anglais
Résumé
LLC-PK1 cells, an established cell line from pig kidney with proximal tubule properties, were cultivated in vitro at confluence on plastic dishes. They were then exposed (apical side) to inorganic cadmium (CdCl2, 5 microM) for periods ranging between 1 to 24 h. Analysis of the cell supernatant after homogenisation and ultracentrifugation indicated that Cd taken up in the first 3 h was bound to cytosolic high molecular weight proteins, but was redistributed to low molecular weight proteins at later stages. Induction of Cd-metallothionein (Cd-Mt) synthesis, as judged from Cd-Mt binding to a specific anti-Cd-Mt antibody and from the rate of 35S-cys incorporation into a specific protein fraction, was apparent 3-6 h after the addition of Cd to the incubation medium.
Mots-clé
Animals, Cadmium/pharmacokinetics, Cell Line, Cytosol/metabolism, Epithelium/metabolism, Kidney/metabolism, Metallothionein/biosynthesis, Protein Biosynthesis, Swine
Pubmed
Web of science
Création de la notice
21/01/2008 12:54
Dernière modification de la notice
20/08/2019 14:09
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