Indirect effects of leptin receptor deficiency on lymphocyte populations and immune response in db/db mice

Détails

ID Serval
serval:BIB_29331B8D2C38
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Indirect effects of leptin receptor deficiency on lymphocyte populations and immune response in db/db mice
Périodique
Journal of Immunology
Auteur⸱e⸱s
Palmer  G., Aurrand-Lions  M., Contassot  E., Talabot-Ayer  D., Ducrest-Gay  D., Vesin  C., Chobaz-Peclat  V., Busso  N., Gabay  C.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
09/2006
Volume
177
Numéro
5
Pages
2899-907
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 1
Résumé
Leptin-deficient ob/ob and leptin receptor (Ob-rb)-deficient db/db mice display a marked thymic atrophy and exhibit defective immune responses. Lymphocytes express leptin receptors and leptin exerts direct effects on T cells in vitro. In addition, ob/ob and db/db mice display multiple neuroendocrine and metabolic defects, through which leptin deficiency may indirectly affect the immune system in vivo. To study the relative contributions of direct and indirect effects of leptin on the immune system in a normal environment, we generated bone marrow chimeras (BMCs) by transplantation of leptin receptor-deficient db/db, or control db/+, bone marrow cells into wild-type (WT) recipients. The size and cellularity of the thymus, as well as cellular and humoral immune responses, were similar in db/db to WT and db/+ to WT BMCs. The immune phenotype of db/db mice is thus not explained by a cell autonomous defect of db/db lymphocytes. Conversely, thymus weight and cell number were decreased in the reverse graft setting in WT to db/db BMCs, indicating that expression of the leptin receptor in the environment is important for T cell development. Finally, normal thymocyte development occurred in fetal db/db thymi transplanted into WT hosts, indicating that direct effects of leptin are not required locally in the thymic microenvironment. In conclusion, direct effects of leptin on bone marrow-derived cells and on thymic stromal cells are not necessary for T lymphocyte maturation in normal mice. In contrast, leptin receptor deficiency affects the immune system indirectly via changes in the systemic environment.
Mots-clé
Animals Lymph Nodes/cytology Lymphocyte Count Lymphocytes/*cytology/*immunology Male Methylation Mice Mice, Knockout Phenotype Receptors, Cell Surface/*deficiency/genetics/*metabolism Serum Albumin, Bovine/chemistry/immunology Thymus Gland/cytology/growth & development/immunology
Pubmed
Web of science
Création de la notice
25/01/2008 8:29
Dernière modification de la notice
20/08/2019 13:08
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