Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions.

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Ressource 1Télécharger: BIB_29253E2B9AA2.P001.pdf (385.19 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_29253E2B9AA2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Response of the Pacific oyster Crassostrea gigas, Thunberg 1793, to pesticide exposure under experimental conditions.
Périodique
Journal of Experimental Biology
Auteur(s)
Collin H., Meistertzheim A.L., David E., Moraga D., Boutet I.
ISSN
1477-9145[electronic], 0022-0949[linking]
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
213
Numéro
23
Pages
4010-4017
Langue
anglais
Résumé
Pesticide run-off into the ocean represents a potential threat to marine organisms, especially bivalves living in coastal environments. However, little is known about the effects of environmentally relevant concentrations of pesticides at the individual level. In this study, the suppression subtractive hybridisation technique was used to discover the main physiological function affected by a cocktail of three pesticides (lindane, metolachlor and carbofuran) in the Pacific oyster Crassostrea gigas. Two oyster populations exposed to different pollution levels in the wild were investigated. The pesticide concentrations used to induce stress were close to those found in the wild. In a time course experiment, the expression of three genes implicated in iron metabolism and oxidative stress as well as that of two ubiquitous stress proteins was examined. No clear regulation of gene or protein expression was found, potentially due to a low-dose effect. However, we detected a strong site- and organ-specific response to the pesticides. This study thus (1) provides insight into bivalve responses to pesticide pollution at the level of the transcriptome, which is the first level of response for organisms facing pollution, and (2) raises interesting questions concerning the importance of the sites and organs studied in the toxicogenomic field.
Mots-clé
SSH library, pesticides, C. gigas, gene expression, oxidative stress, iron metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
08/09/2010 15:27
Dernière modification de la notice
20/08/2019 14:08
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