Evidenziazione degli Helicobacter Pylori in 201 biopsie gastriche mediante Polymerase Chain Reaction, colorazioni istologiche (EE/giemsa) e di immunoistochimica. [Detection of Helicobacter pylori in 201 stomach biopsies using the polymerase chain reaction, histological staining (H&E/Giemsa) and immunohistochemistry]

Détails

ID Serval
serval:BIB_290AEBA4C427
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Evidenziazione degli Helicobacter Pylori in 201 biopsie gastriche mediante Polymerase Chain Reaction, colorazioni istologiche (EE/giemsa) e di immunoistochimica. [Detection of Helicobacter pylori in 201 stomach biopsies using the polymerase chain reaction, histological staining (H&E/Giemsa) and immunohistochemistry]
Périodique
Pathologica
Auteur⸱e⸱s
Casazza  S., Tunesi  G., Marinaro  E., Caruso  F., Canepa  M., Michetti  P., Rovida  S.
ISSN
0031-2983 (Print)
Statut éditorial
Publié
Date de publication
08/1997
Volume
89
Numéro
4
Pages
405-11
Notes
Comparative Study
English Abstract
Journal Article --- Old month value: Aug
Résumé
Helicobacter Pylori is believed to be an essential etiologic agent of type B chronic gastritis and peptic ulcer disease in humans. Recent reports have also suggested a role for these organisms in the development of gastric carcinoma and MALT-Lymphoma. A variety of diagnostic procedures are used for the identification of Helicobacter Pylori in clinical samples. 201 gastric biopsy (164 antral and 37 body biopsy specimens) were obtained from 164 patients with endoscopic abnormalities. These samples were studied for the detection of the presence of Helicobacter Pylori by histological staining (EE/Giemsa), immunohistochemistry and PCR by using a primer pair derived from the nucleotide sequence of the Urease A gene of Helicobacter Pylori. Specific amplification of a 411 base pair DNA fragment from all strain of Helicobacter Pylori tested was achieved. Of the 201 gastric biopsy analyzed, 63 (31%) were infected with Helicobacter Pylori on the basis of both histological and immunohistochemical staining, and 81 (41%) were positive with PCR (P < 0.001). Our results support a role for PCR in the rapid and highly sensitive and specific identification of Helicobacter Pylori in gastric biopsy specimens.
Mots-clé
Azure Stains Biopsy *Coloring Agents DNA, Bacterial/isolation & purification Eosine Yellowish-(YS) Evaluation Studies Female Gastric Mucosa/*microbiology/pathology Gastritis/epidemiology/*microbiology/pathology Helicobacter Infections/epidemiology/*microbiology/pathology Helicobacter pylori/genetics/immunology/*isolation & purification Hematoxylin Humans *Immunoenzyme Techniques Male *Polymerase Chain Reaction Pyloric Antrum/microbiology Sensitivity and Specificity
Pubmed
Création de la notice
25/01/2008 17:02
Dernière modification de la notice
20/08/2019 14:08
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