Collective Resistance in Microbial Communities by Intracellular Antibiotic Deactivation.

Détails

Ressource 1Télécharger: journal.pbio.2000631.pdf (1769.16 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_28FDBC828ACF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Collective Resistance in Microbial Communities by Intracellular Antibiotic Deactivation.
Périodique
PLoS biology
Auteur(s)
Sorg R.A., Lin L., van Doorn G.S., Sorg M., Olson J., Nizet V., Veening J.W.
ISSN
1545-7885 (Electronic)
ISSN-L
1544-9173
Statut éditorial
Publié
Date de publication
12/2016
Peer-reviewed
Oui
Volume
14
Numéro
12
Pages
e2000631
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
The structure and composition of bacterial communities can compromise antibiotic efficacy. For example, the secretion of β-lactamase by individual bacteria provides passive resistance for all residents within a polymicrobial environment. Here, we uncover that collective resistance can also develop via intracellular antibiotic deactivation. Real-time luminescence measurements and single-cell analysis demonstrate that the opportunistic human pathogen Streptococcus pneumoniae grows in medium supplemented with chloramphenicol (Cm) when resistant bacteria expressing Cm acetyltransferase (CAT) are present. We show that CAT processes Cm intracellularly but not extracellularly. In a mouse pneumonia model, more susceptible pneumococci survive Cm treatment when coinfected with a CAT-expressing strain. Mathematical modeling predicts that stable coexistence is only possible when antibiotic resistance comes at a fitness cost. Strikingly, CAT-expressing pneumococci in mouse lungs were outcompeted by susceptible cells even during Cm treatment. Our results highlight the importance of the microbial context during infectious disease as a potential complicating factor to antibiotic therapy.

Mots-clé
Anti-Bacterial Agents/pharmacology, Drug Resistance, Microbial, Streptococcus pneumoniae/drug effects
Pubmed
Open Access
Oui
Création de la notice
03/01/2017 19:12
Dernière modification de la notice
20/08/2019 14:08
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