Role of the sub-cellular localization of RasGAP fragment N2 for its ability to sensitize cancer cells to genotoxin-induced apoptosis.

Détails

ID Serval
serval:BIB_28AE25FFC0CD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Role of the sub-cellular localization of RasGAP fragment N2 for its ability to sensitize cancer cells to genotoxin-induced apoptosis.
Périodique
Experimental Cell Research
Auteur(s)
Annibaldi A., Michod D., Vanetta L., Cruchet S., Nicod P., Dubuis G., Bonvin C., Widmann C.
ISSN
1090-2422[electronic]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
315
Numéro
12
Pages
2081-2091
Langue
anglais
Résumé
The specific sensitization of tumor cells to the apoptotic response induced by genotoxins is a promising way of increasing the efficacy of chemotherapies. The RasGAP-derived fragment N2, while not regulating apoptosis in normal cells, potently sensitizes tumor cells to cisplatin- and other genotoxin-induced cell death. Here we show that fragment N2 in living cells is mainly located in the cytoplasm and only minimally associated with specific organelles. The cytoplasmic localization of fragment N2 was required for its cisplatin-sensitization property because targeting it to the mitochondria or the ER abrogated its ability to increase the death of tumor cells in response to cisplatin. These results indicate that fragment N2 requires a spatially constrained cellular location to exert its anti-cancer activity.
Pubmed
Web of science
Création de la notice
19/05/2009 9:10
Dernière modification de la notice
20/08/2019 14:08
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