A rapid microbiopsy system to improve the preservation of biological samples prior to high-pressure freezing.
Détails
ID Serval
serval:BIB_28885
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A rapid microbiopsy system to improve the preservation of biological samples prior to high-pressure freezing.
Périodique
Journal of Microscopy
ISSN
0022-2720
Statut éditorial
Publié
Date de publication
2003
Volume
212
Numéro
Pt 1
Pages
3-12
Langue
anglais
Notes
Old uritopublisher value http://www.blackwell-synergy.com/Journals/member/institutions/issuelist.asp?journal=jmi
Résumé
A microbiopsy system for fast excision and transfer of biological specimens from donor to high-pressure freezer was developed. With a modified, commercially available, Promag 1.2 biopsy gun, tissue samples can be excised with a size small enough (0.6 mm x 1.2 mm x 0.3 mm) to be easily transferred into a newly designed specimen platelet. A self-made transfer unit allows fast transfer of the specimen from the needle into the specimen platelet. The platelet is then fixed in a commercially available specimen holder of a high-pressure freezing machine (EM PACT, Leica Microsystems, Vienna, Austria) and frozen therein. The time required by a well-instructed (but not experienced) person to execute all steps is in the range of half a minute. This period is considered short enough to maintain the excised tissue pieces close to their native state. We show that a range of animal tissues (liver, brain, kidney and muscle) are well preserved. To prove the quality of freezing achieved with the system, we show vitrified ivy leaves high-pressure frozen in the new specimen platelet.
Mots-clé
Animals, Biopsy, Brain, Cryopreservation, Cryoultramicrotomy, Freeze Substitution, Freezing, Kidney, Liver, Microscopy, Electron, Muscles, Plant Leaves, Pressure, Rats, Specimen Handling, Time Factors
OAI-PMH
Pubmed
Web of science
Création de la notice
19/11/2007 13:26
Dernière modification de la notice
20/08/2019 14:08