Neurotrophin receptor-mediated death of misspecified neurons generated from embryonic stem cells lacking Pax6.

Détails

ID Serval
serval:BIB_287FC3E07845
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Neurotrophin receptor-mediated death of misspecified neurons generated from embryonic stem cells lacking Pax6.
Périodique
Cell stem cell
Auteur⸱e⸱s
Nikoletopoulou V., Plachta N., Allen N.D., Pinto L., Götz M., Barde Y.A.
ISSN
1934-5909 (Print)
ISSN-L
1875-9777
Statut éditorial
Publié
Date de publication
11/2007
Peer-reviewed
Oui
Volume
1
Numéro
5
Pages
529-540
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Pax6-positive radial glial (RG) cells are the progenitors of most glutamatergic neurons in the cortex, a lineage that can be recapitulated in vitro using embryonic stem (ES) cells. We show here that ES cells lacking Pax6, a transcription factor long known to be essential for cortical development, generate Mash1-positive RG cells that differentiate in GABAergic neurons. These neurons express high levels of the neurotrophin receptor p75NTR causing their rapid death. Pax6 function was also investigated following transplantation of ES cells in the developing chick telencephalon and in mice lacking both Pax6 and p75NTR. Taken together, our results indicate that reliable predictions can be made with cultured ES cells when used to explore the role of genes impacting early aspects of mammalian neurogenesis. They also provide a novel opportunity to compare the molecular constituents of glutamatergic with those of GABA-ergic neurons and to explore the mechanisms of their generation.
Mots-clé
Animals, Basic Helix-Loop-Helix Transcription Factors/metabolism, Cell Death, Cell Differentiation, Cell Line, Cell Lineage, Cerebral Cortex/embryology, Cerebral Cortex/metabolism, Chick Embryo, Embryonic Stem Cells/drug effects, Embryonic Stem Cells/metabolism, Embryonic Stem Cells/pathology, Embryonic Stem Cells/transplantation, Eye Proteins/genetics, Eye Proteins/metabolism, Gestational Age, Glutamic Acid/metabolism, Homeodomain Proteins/genetics, Homeodomain Proteins/metabolism, Mice, Mice, Knockout, Neuroglia/drug effects, Neuroglia/metabolism, Neuroglia/pathology, Neuroglia/transplantation, Neurons/drug effects, Neurons/metabolism, Neurons/pathology, Neurons/transplantation, PAX6 Transcription Factor, Paired Box Transcription Factors/deficiency, Paired Box Transcription Factors/genetics, Paired Box Transcription Factors/metabolism, Phenotype, Receptors, Nerve Growth Factor/deficiency, Receptors, Nerve Growth Factor/genetics, Receptors, Nerve Growth Factor/metabolism, Repressor Proteins/genetics, Repressor Proteins/metabolism, Telencephalon/embryology, Telencephalon/metabolism, Transfection, Tretinoin/pharmacology, gamma-Aminobutyric Acid/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
04/06/2024 10:49
Dernière modification de la notice
05/06/2024 9:05
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