Importance of amoebae as a tool to isolate amoeba-resisting microorganisms and for their ecology and evolution: the Chlamydia paradigm.

Détails

Ressource 1Télécharger: BIB_282B2BF97810.P001.pdf (1393.90 [Ko])
Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_282B2BF97810
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Importance of amoebae as a tool to isolate amoeba-resisting microorganisms and for their ecology and evolution: the Chlamydia paradigm.
Périodique
Environmental Microbiology Reports
Auteur⸱e⸱s
Kebbi-Beghdadi C., Greub G.
ISSN
1758-2229 (Electronic)
ISSN-L
1758-2229
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
6
Numéro
4
Pages
309-324
Langue
anglais
Résumé
Free-living amoebae are distributed worldwide and are frequently in contact with humans and animals. As cysts, they can survive in very harsh conditions and resist biocides and most disinfection procedures. Several microorganisms, called amoeba-resisting microorganisms (ARMs), have evolved to survive and multiply within these protozoa. Among them are many important pathogens, such as Legionella and Mycobacteria, and also several newly discovered Chlamydia-related bacteria, such as Parachlamydia acanthamoebae, Estrella lausannensis, Simkania negevensis or Waddlia chondrophila whose pathogenic role towards human or animal is strongly suspected. Amoebae represent an evolutionary crib for their resistant microorganisms since they can exchange genetic material with other ARMs and develop virulence traits that will be further used to infect other professional phagocytes. Moreover, amoebae constitute an ideal tool to isolate strict intracellular microorganisms from complex microbiota, since they will feed on other fast-growing bacteria, such as coliforms potentially present in the investigated samples. The paradigm that ARMs are likely resistant to macrophages, another phagocytic cell, and that they are likely virulent towards humans and animals is only partially true. Indeed, we provide examples of the Chlamydiales order that challenge this assumption and suggest that the ability to multiply in protozoa does not strictly correlate with pathogenicity and that we should rather use the ability to replicate in multiple and diverse eukaryotic cells as an indirect marker of virulence towards mammals. Thus, cell-culture-based microbial culturomics should be used in the future to try to discover new pathogenic bacterial species.
Mots-clé
Amoeba/microbiology, Animals, Chlamydia/growth & development, Ecosystem, Humans, Legionella/growth & development, Macrophages/microbiology, Mycobacterium/growth & development, Phagocytosis
Pubmed
Web of science
Création de la notice
03/04/2016 21:17
Dernière modification de la notice
20/08/2019 14:07
Données d'usage