The lymphoid past of mouse plasmacytoid cells and thymic dendritic cells.
Détails
ID Serval
serval:BIB_28039
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The lymphoid past of mouse plasmacytoid cells and thymic dendritic cells.
Périodique
Journal of Immunology
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Statut éditorial
Publié
Date de publication
2003
Volume
170
Numéro
10
Pages
4926-4932
Langue
anglais
Résumé
There has been controversy over the possible lymphoid origin of certain dendritic cell (DC) subtypes. To resolve this issue, DC and plasmacytoid pre-DC isolated from normal mouse tissues were analyzed for transient (mRNA) and permanent (DNA rearrangement) markers of early stages of lymphoid development. About 27% of the DNA of CD8(+) DC from thymus, and 22-35% of the DNA of plasmacytoid pre-DC from spleen and thymus, was found to contain IgH gene D-J rearrangements, compared with 40% for T cells. However, the DC DNA did not contain IgH gene V-D-J rearrangements nor T cell Ag receptor beta gene D-J rearrangements. The same DC lineage populations containing IgH D-J rearrangements expressed mRNA for CD3 chains, and for pre-T alpha. In contrast, little of the DNA of the conventional DC derived from spleen, lymph nodes, or skin, whether CD8(+) or CD8(-), contained IgH D-J rearrangements and splenic conventional DC expressed very little CD3 epsilon or pre-T alpha mRNA. Therefore, many plasmacytoid pre-DC and thymic CD8(+) DC have shared early steps of development with the lymphoid lineages, and differ in origin from conventional peripheral DC.
Mots-clé
Animals, Antigens, CD3, Bone Marrow Cells/immunology, Bone Marrow Cells/metabolism, Bone Marrow Transplantation, Cell Differentiation/genetics, Cell Differentiation/immunology, Cell Lineage/genetics, Cell Lineage/immunology, Cells, Cultured, Complementarity Determining Regions/biosynthesis, Complementarity Determining Regions/genetics, Dendritic Cells/cytology, Dendritic Cells/immunology, Female, Gene Rearrangement, B-Lymphocyte, Heavy Chain, Genes, T-Cell Receptor alpha, Lymphoid Tissue/cytology, Lymphoid Tissue/immunology, Male, Membrane Glycoproteins/biosynthesis, Membrane Glycoproteins/genetics, Mice, Mice, Inbred C57BL, Mice, Transgenic, Phagocytosis/genetics, Phagocytosis/immunology, Plasma Cells/cytology, Plasma Cells/immunology, RNA, Messenger/biosynthesis, Radiation Chimera/immunology, Receptors, Antigen, T-Cell/biosynthesis, Receptors, Antigen, T-Cell/genetics, Receptors, Antigen, T-Cell, alpha-beta, Stem Cells/cytology, Stem Cells/immunology, Thymus Gland/cytology, Thymus Gland/immunology
OAI-PMH
Pubmed
Web of science
Création de la notice
19/11/2007 13:24
Dernière modification de la notice
20/08/2019 14:07