Positive control of swarming, rhamnolipid synthesis, and lipase production by the posttranscriptional RsmA/RsmZ system in Pseudomonas aeruginosa PAO1.

Détails

ID Serval
serval:BIB_277689FC2EC3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Positive control of swarming, rhamnolipid synthesis, and lipase production by the posttranscriptional RsmA/RsmZ system in Pseudomonas aeruginosa PAO1.
Périodique
Journal of Bacteriology
Auteur(s)
Heurlier K., Williams F., Heeb S., Dormond C., Pessi G., Singer D., Cámara M., Williams P., Haas D.
ISSN
0021-9193[print], 0021-9193[linking]
Statut éditorial
Publié
Date de publication
2004
Volume
186
Numéro
10
Pages
2936-2945
Langue
anglais
Résumé
In Pseudomonas aeruginosa, the small RNA-binding, regulatory protein RsmA is a negative control element in the formation of several extracellular products (e.g., pyocyanin, hydrogen cyanide, PA-IL lectin) as well as in the production of N-acylhomoserine lactone quorum-sensing signal molecules. RsmA was found to control positively the ability to swarm and to produce extracellular rhamnolipids and lipase, i.e., functions contributing to niche colonization by P. aeruginosa. An rsmA null mutant was entirely devoid of swarming but produced detectable amounts of rhamnolipids, suggesting that factors in addition to rhamnolipids influence the swarming ability of P. aeruginosa. A small regulatory RNA, rsmZ, which antagonized the effects of RsmA, was identified in P. aeruginosa. Expression of the rsmZ gene was dependent on both the global regulator GacA and RsmA, increased with cell density, and was subject to negative autoregulation. Overexpression of rsmZ and a null mutation in rsmA resulted in quantitatively similar, negative or positive effects on target genes, in agreement with a model that postulates titration of RsmA protein by RsmZ RNA.
Mots-clé
Amino Acid Sequence, Bacterial Proteins/antagonists & inhibitors, Bacterial Proteins/physiology, Base Sequence, Cloning, Molecular, Gene Expression Regulation, Bacterial, Glycolipids/biosynthesis, Lipase/biosynthesis, Molecular Sequence Data, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/metabolism, RNA, Bacterial/physiology, RNA-Binding Proteins/antagonists & inhibitors, RNA-Binding Proteins/physiology, Repressor Proteins/antagonists & inhibitors, Repressor Proteins/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 18:01
Dernière modification de la notice
20/08/2019 14:06
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