Use of transcriptome data to unravel the fine structure of genes involved in sepsis.

Détails

ID Serval
serval:BIB_27587
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Use of transcriptome data to unravel the fine structure of genes involved in sepsis.
Périodique
Journal of Infectious Diseases
Auteur⸱e⸱s
Stevenson B.J., Iseli C., Beutler B., Jongeneel C.V.
ISSN
0022-1899[print], 0022-1899[linking]
Statut éditorial
Publié
Date de publication
2003
Volume
187
Numéro
Suppl. 2
Pages
S308-S314
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The sequence of the human genome is providing researchers with the scaffold upon which genes are built. The definition of the boundaries of the genes themselves and of their complex architecture requires a mapping of the transcriptome to the genome. A methodology was developed for generating a detailed transcriptome map and for reconstituting transcripts by using the genome as a template. As a demonstration of the potential of this method, the structure of the human Toll-like receptor (TLR) genes was reevaluated. For all TLR genes for which a genomic sequence was available (i.e., all except TLR10), novel features of the gene structure were discovered. These features include multiple alternative polyadenylation sites, additional exons or splice variants, and overlaps with other genes. These findings have implications for the analysis of TLR gene expression and for the diversity of the proteins encoded by these genes.
Mots-clé
Gene Expression Profiling, Genome, Human, Humans, Membrane Glycoproteins/genetics, RNA, Messenger/analysis, RNA, Messenger/genetics, Receptors, Cell Surface/genetics, Sepsis/genetics, Toll-Like Receptor 10, Toll-Like Receptors, Transcription, Genetic/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 10:54
Dernière modification de la notice
20/08/2019 14:06
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