Dual function of a nuclear factor I binding site in MMTV transcription regulation

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_2745AC5445E1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Dual function of a nuclear factor I binding site in MMTV transcription regulation
Périodique
Nucleic Acids Research
Auteur(s)
Buetti E., Kuhnel B., Diggelmann H.
ISSN
0305-1048 (Print)
Statut éditorial
Publié
Date de publication
04/1989
Volume
17
Numéro
8
Pages
3065-78
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Apr 25
Résumé
Using linker-scanning mutagenesis we had previously identified four elements within the MMTV LTR which are necessary for transcriptional stimulation by glucocorticoid hormones. Two of them overlapped with regions to which the glucocorticoid receptor binds in vitro. The third element contained a NF-I binding site, and the fourth the TATA box. Here we show that mutations that abolish in vitro binding of NF-I had a negative effect also on the basal activity of the MMTV promoter of LTR-containing plasmids stably integrated in Ltk- fibroblasts. The analysis of double mutants altered in the NF-I plus either one of the receptor binding elements further demonstrated that the NF-I site functionally cooperated with the proximal (-120) element, which alone was extremely inefficient in stimulation. The stronger distal (-181/-172) element was independent of NF-I and showed functional cooperativity with the proximal hormone-binding element.
Mots-clé
Animals Binding Sites *CCAAT-Enhancer-Binding Proteins DNA Mutational Analysis DNA-Binding Proteins/*physiology Dexamethasone/pharmacology Gene Expression Regulation/drug effects L Cells (Cell Line) Mammary Tumor Virus, Mouse/*genetics Mice NFI Transcription Factors Nuclear Proteins/*physiology *Promoter Regions (Genetics) Receptors, Glucocorticoid/physiology *Regulatory Sequences, Nucleic Acid Transcription Factors/*physiology Transcription, Genetic Y-Box-Binding Protein 1
Pubmed
Web of science
Création de la notice
25/01/2008 15:25
Dernière modification de la notice
20/08/2019 14:06
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