Expression of an exogenous growth hormone gene by transplantable human epidermal cells
Détails
ID Serval
serval:BIB_2729ADD1D153
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Expression of an exogenous growth hormone gene by transplantable human epidermal cells
Périodique
Science
ISSN
0036-8075 (Print)
Statut éditorial
Publié
Date de publication
09/1987
Volume
237
Numéro
4821
Pages
1476-9
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep 18
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep 18
Résumé
Retrovirus-mediated gene transfer was used to introduce a recombinant human growth hormone gene into cultured human keratinocytes. The transduced keratinocytes secreted biologically active growth hormone into the culture medium. When grafted as an epithelial sheet onto athymic mice, these cultured keratinocytes reconstituted an epidermis that was similar in appearance to that resulting from normal cells, but from which human growth hormone could be extracted. Transduced epidermal cells may prove to be a general vehicle for the delivery of gene products by means of grafting.
Mots-clé
DNA, Recombinant/metabolism
Epidermis/*metabolism
*Gene Expression Regulation
Growth Hormone/*genetics
Humans
Retroviridae/genetics
Transcription, Genetic
Transduction, Genetic
Pubmed
Web of science
Création de la notice
28/01/2008 9:41
Dernière modification de la notice
20/08/2019 14:06