This study compared a new disposable silicone eyecup for high-frequency ultrasound biomicroscopy to the standard hard plastic eyecup. The results showed the silicone eyecup attenuated leakage of the medium in which the probe was immersed, decreased scanning time, decreased distortion of the iris and angle during scanning, enhanced image quality, and improved patient comfort. These findings indicate the silicone eyecup is a good alternative to the hard plastic eyecup and also is easier to use.