Deletion of GEL2 encoding for a beta(1-3)glucanosyltransferase affects morphogenesis and virulence in Aspergillus fumigatus

Détails

ID Serval
serval:BIB_26ECB3E93BAF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Deletion of GEL2 encoding for a beta(1-3)glucanosyltransferase affects morphogenesis and virulence in Aspergillus fumigatus
Périodique
Molecular Microbiology
Auteur(s)
Mouyna  I., Morelle  W., Vai  M., Monod  M., Lechenne  B., Fontaine  T., Beauvais  A., Sarfati  J., Prevost  M. C., Henry  C., Latge  J. P.
ISSN
0950-382X (Print)
Statut éditorial
Publié
Date de publication
06/2005
Volume
56
Numéro
6
Pages
1675-88
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
The first fungal glycosylphosphatidylinositol anchored beta(1-3)glucanosyltranferase (Gel1p) has been described in Aspergillus fumigatus and its encoding gene GEL1 identified. Glycosylphosphatidylinositol-anchored glucanosyltransferases play an active role in the biosynthesis of the fungal cell wall. We characterize here GEL2, a homologue of GEL1. Both homologues share common characteristics: (i) GEL1 and GEL2 are constitutively expressed during over a range of growth conditions; (ii) Gel2p is also a putative GPI-anchored protein and shares the same beta(1-3)glucanosyltransferase activity as Gel1p and (iii) GEL2, like GEL1, is able to complement the Deltagas1 deletion in Saccharomyces cerevisiae confirming that Gelp and Gasp have the same enzymatic activity. However, disruption of GEL1 did not result in a phenotype whereas a Deltagel2 mutant and the double mutant Deltagel1Deltagel2 exhibit slower growth, abnormal conidiogenesis, and an altered cell wall composition. In addition, the Deltagel2 and the Deltagel1Deltagel2 mutant have reduced virulence in a murine model of invasive aspergillosis. These data suggest for the first time that beta(1-3)glucanosyltransferase activity is required for both morphogenesis and virulence in A. fumigatus.
Mots-clé
Animals Aspergillosis/*microbiology Aspergillus fumigatus/enzymology/genetics/*growth & development/*pathogenicity Female Fungal Proteins/genetics/metabolism Gene Deletion Genetic Complementation Test Glucan Endo-1,3-beta-D-Glucosidase/*genetics/metabolism Mice Molecular Sequence Data *Morphogenesis Phenotype Sequence Analysis, DNA Transformation, Genetic Virulence
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:47
Dernière modification de la notice
20/08/2019 13:05
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