Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line.

Détails

ID Serval
serval:BIB_26B11CD9D133
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line.
Périodique
Biotechnology letters
Auteur(s)
Shen X., Dojcinovic D., Baldi L., Hacker D.L., Luescher I.F., Wurm F.M.
ISSN
1573-6776 (Electronic)
ISSN-L
0141-5492
Statut éditorial
Publié
Date de publication
01/2018
Peer-reviewed
Oui
Volume
40
Numéro
1
Pages
85-92
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
To investigate the effects of operational process conditions on expression of MHC class II protein from a stable Drosophila S2 cell line.
When the Drosophila S2 cells were grown in vented orbitally shaken TubeSpin bioreactor 600 containers, cell growth was improved three-fold and the yield of recombinant major histocompatibility (MHC) class II protein (HLA-DR1 javax.xml.bind.JAXBElement@1b28618d ) increased four-fold over the levels observed for the same cells cultivated in roller bottles (RB) without vented caps. Culturing in RB with vented caps while increasing the rotation speed from 6 rpm to 18 rpm also improved cell growth five-fold and protein productivity three-fold which is comparable to the levels observed in the orbitally shaken containers. Protein activity was found to be almost identical between the two vessel systems tested.
Optimized cell culture conditions and a more efficient vessel type can enhance gas transfer and mixing and lead to substantial improvement of recombinant product yields from S2 cells.

Mots-clé
Orbital shaking, Recombinant protein, Roller bottle, S2 cells, Tetramer technology
Pubmed
Web of science
Création de la notice
19/10/2017 8:01
Dernière modification de la notice
21/08/2019 5:34
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