Cryo-electron microscopy of vitrified chromosomes in situ

Détails

ID Serval
serval:BIB_26AD935F37C5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cryo-electron microscopy of vitrified chromosomes in situ
Périodique
EMBO Journal
Auteur(s)
McDowall  A. W., Smith  J. M., Dubochet  J.
ISSN
0261-4189 (Print)
Statut éditorial
Publié
Date de publication
06/1986
Volume
5
Numéro
6
Pages
1395-402
Notes
Journal Article --- Old month value: Jun
Résumé
Chromosomes of metaphase-arrested Chinese hamster ovary (CHO) and HeLa cells were examined in situ, unfixed and unstained, by cryo-electron microscopy. In hydrated, vitrified cryo-sections, chromosomes exhibit a characteristic homogeneous, grainy texture, which, on optical diffraction, gives rise to a broad reflection corresponding to 11 nm. No superstructure or periodic order is discernible. These observations suggest that the chromosome is formed by the compact association of 11 nm filaments, or portions thereof, interacting in a manner akin to the molecules of a liquid. Some implications of the liquid model of chromosome structure are discussed.
Mots-clé
Animals Cell Line Chromosomes/*ultrastructure Chromosomes, Human/*ultrastructure Cricetinae Cricetulus Female Freezing Hela Cells/ultrastructure Humans Male Metaphase Microscopy, Electron Ovary
Pubmed
Web of science
Création de la notice
24/01/2008 10:25
Dernière modification de la notice
20/08/2019 13:05
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