Adenylate cyclase 1 as a key actor in the refinement of retinal projection maps
Détails
ID Serval
serval:BIB_262FB38DD7DA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Adenylate cyclase 1 as a key actor in the refinement of retinal projection maps
Périodique
Journal of Neuroscience
ISSN
1529-2401 (Electronic)
Statut éditorial
Publié
Date de publication
03/2003
Volume
23
Numéro
6
Pages
2228-38
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 15
Research Support, Non-U.S. Gov't --- Old month value: Mar 15
Résumé
cAMP occupies a strategic position to control neuronal responses to a large variety of developmental cues. We have analyzed the role of calcium-stimulated adenylate cyclase 1 (AC1) in the development of retinal topographic maps. AC1 is expressed in retinal ganglion cells (RGCs) from embryonic day 15 to adulthood with a peak during the first postnatal week. At that time, the other calcium-stimulated AC, AC8, is expressed in the superior colliculus (SC) but not in the RGCs. In mice of the barrelless strain, which carry an inactivating mutation of the AC1 gene, calcium-stimulated AC activity is reduced by 40-60% in the SC and retina. RGC projection maps were analyzed with a variety of anterograde and retrograde tracers. After an initially normal development until postnatal day 3, retinal fibers from the ipsilateral and contralateral eye fail to segregate into eye-specific domains in the lateral geniculate nucleus and the SC. Topographic defects in the fine tuning of the retinotectal and retinogeniculate maps are also observed with abnormalities in the confinement of the retinal axon arbors in the anteroposterior and mediolateral dimensions. This is attributable to the lack of elimination of misplaced axon collaterals and to the maintenance of a transient ipsilateral projection. These results establish an essential role of AC1 in the fine patterning of the retinal map. Calcium-modulated cAMP production in the RGCs could constitute an important link between activity-dependent changes and the anatomical restructuring of the retinal terminal arbors within central targets.
Mots-clé
Adenylate Cyclase/genetics/*physiology
Animals
Axons/enzymology/physiology
Cell Count
Geniculate Bodies/cytology/embryology/enzymology/growth & development
In Situ Hybridization
Isoenzymes/genetics/physiology
Mice
Mice, Neurologic Mutants
RNA, Messenger/analysis/biosynthesis
Retina/cytology/*enzymology
Retinal Ganglion Cells/cytology/enzymology
Superior Colliculus/cytology/embryology/enzymology/growth & development
Visual Pathways/cytology/embryology/enzymology/growth & development
Pubmed
Web of science
Création de la notice
24/01/2008 14:40
Dernière modification de la notice
20/08/2019 13:04