SWR1 and INO80 chromatin remodelers contribute to DNA double-strand break perinuclear anchorage site choice.

Détails

Ressource 1Télécharger: BIB_2591306D968C.P001.pdf (1603.92 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_2591306D968C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
SWR1 and INO80 chromatin remodelers contribute to DNA double-strand break perinuclear anchorage site choice.
Périodique
Molecular Cell
Auteur(s)
Horigome C., Oma Y., Konishi T., Schmid R., Marcomini I., Hauer M.H., Dion V., Harata M., Gasser S.M.
ISSN
1097-4164 (Electronic)
ISSN-L
1097-2765
Statut éditorial
Publié
Date de publication
2014
Volume
55
Numéro
4
Pages
626-639
Langue
anglais
Résumé
Persistent DNA double-strand breaks (DSBs) are recruited to the nuclear periphery in budding yeast. Both the Nup84 pore subcomplex and Mps3, an inner nuclear membrane (INM) SUN domain protein, have been implicated in DSB binding. It was unclear what, if anything, distinguishes the two potential sites of repair. Here, we characterize and distinguish the two binding sites. First, DSB-pore interaction occurs independently of cell-cycle phase and requires neither the chromatin remodeler INO80 nor recombinase Rad51 activity. In contrast, Mps3 binding is S and G2 phase specific and requires both factors. SWR1-dependent incorporation of Htz1 (H2A.Z) is necessary for break relocation to either site in both G1- and S-phase cells. Importantly, functional assays indicate that mutations in the two sites have additive repair defects, arguing that the two perinuclear anchorage sites define distinct survival pathways.
Mots-clé
UI="D000251">Adenosine Triphosphatases/UI="Q000502">physiology, UI="D001665">Binding Sites/UI="Q000235">genetics, UI="D001665">Binding Sites/UI="Q000502">physiology, UI="D002453">Cell Cycle/UI="Q000235">genetics, UI="D002453">Cell Cycle/UI="Q000502">physiology, UI="D042002">Chromatin Assembly and Disassembly/UI="Q000235">genetics, UI="D042002">Chromatin Assembly and Disassembly/UI="Q000502">physiology, UI="D053903">DNA Breaks, Double-Stranded, UI="D004271">DNA, Fungal/UI="Q000235">genetics, UI="D005656">Fungal Proteins/UI="Q000502">physiology, UI="D006657">Histones/UI="Q000378">metabolism, UI="D008565">Membrane Proteins/UI="Q000378">metabolism, UI="D009154">Mutation, UI="D004718">Saccharomycetales/UI="Q000235">genetics, UI="D004718">Saccharomycetales/UI="Q000378">metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
16/12/2014 15:23
Dernière modification de la notice
20/08/2019 14:04
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