Molecular characterization of the food-borne fungus Neosartorya fischeri (Malloch and Cain)

Détails

ID Serval
serval:BIB_25446A7B5CD7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Molecular characterization of the food-borne fungus Neosartorya fischeri (Malloch and Cain)
Périodique
Applied and Environmental Microbiology
Auteur(s)
Girardin  H., Monod  M., Latge  J. P.
ISSN
0099-2240 (Print)
Statut éditorial
Publié
Date de publication
04/1995
Volume
61
Numéro
4
Pages
1378-83
Notes
Comparative Study
Journal Article --- Old month value: Apr
Résumé
The food-borne fungus Neosartorya fischeri, which is phenotypically related to the human opportunistic pathogen Aspergillus fumigatus, causes spoilage of heat-processed fruit products. Genomic methods were used to type N. fischeri strains and identify the genomic relationship between A. fumigatus and N. fischeri and between the different varieties of N. fischeri. EcoRI restriction fragment length polymorphism (RFLP) patterns obtained after ethidium bromide staining could differentiate most of N. fischeri var. glabra and N. fischeri var. spinosa strains. On the contrary, all N. fischeri var. fischeri strains tested exhibit the same RFLP pattern, which was similar to the A. fumigatus pattern. Similarly, Southern hybridization with a ribosomal probe showed some polymorphism between N. fischeri var. glabra and N. fischeri var. spinosa strains but could not distinguish between N. fischeri var. fischeri and A. fumigatus strains. By using the endonucleases EcoRI, HindIII, and BglII to generate Southern blot patterns with a fragment of the A. fumigatus gene coding for a 33-kDa protease, it was possible to differentiate N. fischeri var. fischeri from A. fumigatus. The difference between N. fischeri and A. fumigatus was confirmed by the use of moderately repetitive nonribosomal A. fumigatus sequences. These results are in agreement with previous studies that showed important infraspecific polymorphism within N. fischeri var. glabra and N. fischeri var. spinosa and, in contrast, the homogeneity of N. fischeri var. fischeri strains. A unique Southern blot pattern was seen for each strain of N. fischeri fingerprinted with the A. fumigatus repetitive sequence.(ABSTRACT TRUNCATED AT 250 WORDS)
Mots-clé
Ascomycota/classification/*genetics/isolation & purification Aspergillus fumigatus/classification/genetics Blotting, Southern DNA Fingerprinting DNA, Fungal/genetics/isolation & purification Endopeptidases/genetics *Food Microbiology Nucleic Acid Hybridization Phenotype Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid Species Specificity Variation (Genetics)
Pubmed
Web of science
Création de la notice
25/01/2008 17:46
Dernière modification de la notice
20/08/2019 14:03
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