Expression of Molecular Differentiation Markers Does Not Correlate with Histological Differentiation Grade in Intrahepatic Cholangiocarcinoma.

Détails

Ressource 1Télécharger: BIB_24C261E7E6AD.P001.pdf (12894.18 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_24C261E7E6AD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Expression of Molecular Differentiation Markers Does Not Correlate with Histological Differentiation Grade in Intrahepatic Cholangiocarcinoma.
Périodique
PloS one
Auteur(s)
Demarez C., Hubert C., Sempoux C., Lemaigre F.P.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
11
Numéro
6
Pages
e0157140
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
The differentiation status of tumor cells, defined by histomorphological criteria, is a prognostic factor for survival of patients affected with intrahepatic cholangiocarcinoma (ICC). To strengthen the value of morphological differentiation criteria, we wished to correlate histopathological differentiation grade with expression of molecular biliary differentiation markers and of microRNAs previously shown to be dysregulated in ICC. We analysed a series of tumors that were histologically classified as well, moderately or poorly differentiated, and investigated the expression of cytokeratin 7, 19 and 903 (CK7, CK19, CK903), SRY-related HMG box transcription factors 4 and 9 (SOX4, SOX9), osteopontin (OPN), Hepatocyte Nuclear Factor-1 beta (HNF1β), Yes-associated protein (YAP), Epithelial cell adhesion molecule (EPCAM), Mucin 1 (MUC1) and N-cadherin (NCAD) by qRT-PCR and immunostaining, and of miR-31, miR-135b, miR-132, miR-200c, miR-221 and miR-222. Unexpectedly, except for subcellular location of SOX9 and OPN, no correlation was found between the expression levels of these molecular markers and histopathological differentiation grade. Therefore, our data point toward necessary caution when investigating the evolution and prognosis of ICC on the basis of cell differentiation criteria.

Mots-clé
Adult, Aged, Bile Duct Neoplasms/genetics, Bile Duct Neoplasms/metabolism, Bile Duct Neoplasms/pathology, Bile Ducts, Intrahepatic/metabolism, Bile Ducts, Intrahepatic/pathology, Biomarkers, Tumor/genetics, Biomarkers, Tumor/metabolism, Cell Differentiation, Cholangiocarcinoma/genetics, Cholangiocarcinoma/metabolism, Cholangiocarcinoma/pathology, Female, Follow-Up Studies, Humans, Male, Middle Aged, Neoplasm Grading, Prognosis
Pubmed
Web of science
Open Access
Oui
Création de la notice
14/06/2016 9:45
Dernière modification de la notice
20/08/2019 14:03
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