Microbiological diagnosis of community-acquired respiratory tract infections by nucleic acid detection.

Détails

ID Serval
serval:BIB_23E58024A6A4
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Microbiological diagnosis of community-acquired respiratory tract infections by nucleic acid detection.
Périodique
Expert Opinion on Medical Diagnostics
Auteur⸱e⸱s
Jaton-Ogay K., Bille J.
ISSN
1753-0059 (Print)
ISSN-L
1753-0059
Statut éditorial
Publié
Date de publication
2008
Volume
2
Numéro
8
Pages
947-961
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish. PDF type: Review
Résumé
Background: Microbiological diagnostic procedures have changed significantly over the last decade. Initially the implementation of the polymerase chain reaction (PCR) resulted in improved detection tests for microbes that were difficult or even impossible to detect by conventional methods such as culture and serology, especially in community-acquired respiratory tract infections (CA-RTI). A further improvement was the development of real-time PCR, which allows end point detection and quantification, and many diagnostic laboratories have now implemented this powerful method. Objective: At present, new performant and convenient molecular tests have emerged targeting in parallel many viruses and bacteria responsible for lower and/or upper respiratory tract infections. The range of test formats and microbial agents detected is evolving very quickly and the added value of these new tests needs to be studied in terms of better use of antibiotics, better patient management, duration of hospitalization and overall costs. Conclusions: Molecular tools for a better microbial documentation of CA-RTI are now available. Controlled studies are now required to address the relevance issue of these new methods, such as, for example, the role of some newly detected respiratory viruses or of the microbial DNA load in a particular patient at a particular time. The future challenge for molecular diagnosis will be to become easy to handle, highly efficient and cost-effective, delivering rapid results with a direct impact on clinical management.
Pubmed
Création de la notice
13/08/2013 11:09
Dernière modification de la notice
20/08/2019 13:01
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