Rev inhibition strongly affects intracellular distribution of human immunodeficiency virus type 1 RNAs.

Détails

ID Serval
serval:BIB_23843
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Rev inhibition strongly affects intracellular distribution of human immunodeficiency virus type 1 RNAs.
Périodique
Journal of virology
Auteur(s)
Cmarko D., Bøe S.O., Scassellati C., Szilvay A.M., Davanger S., Fu X.D., Haukenes G., Kalland K.H., Fakan S.
ISSN
0022-538X (Print)
ISSN-L
0022-538X
Statut éditorial
Publié
Date de publication
10/2002
Peer-reviewed
Oui
Volume
76
Numéro
20
Pages
10473-10484
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
To define the human immunodeficiency virus type 1 (HIV-1) RNA maturation pathways, we analyzed the intracellular distribution of HIV-1 RNA and the viral regulatory proteins Rev and Tat in transfected COS cells and HIV-1-infected lymphoid C8166 cells by means of ultrastructural in situ hybridization using antisense RNA probes and immunoelectron microscopy. The intranuclear viral RNA occurs in ribonucleoprotein fibrils in the perichromatin and interchromatin regions. The simultaneous demonstration of Rev, Tat, Br-labeled RNA, and cellular proteins SC35 and CRM1 in such fibrils reveals the potential of Rev to associate with nascent HIV pre-mRNA and its splicing complex and transport machinery. In a rev-minus system, the env intron-containing, incompletely spliced viral RNAs are revealed only in the nucleus, indicating that Rev is required to initiate the transport to the cytoplasm. Moreover, env intron sequences frequently occur in the periphery of interchromatin granule clusters, while the probe containing the rev exon sequence does not associate with this nucleoplasmic domain. When cells were treated with the CRM1 inhibitor leptomycin B in the presence of Rev protein, the env intron containing HIV RNAs formed clusters throughout the nucleoplasm and accumulated at the nuclear pores. This suggests that Rev is necessary and probably also sufficient for the accumulation of incompletely spliced HIV RNAs at the nuclear pores while CRM1 is needed for translocation across the nuclear pore complex.

Mots-clé
Active Transport, Cell Nucleus, Animals, COS Cells, Cell Nucleus/metabolism, Cercopithecus aethiops, Exons, Gene Products, rev/genetics, Gene Products, rev/metabolism, Gene Products, tat/genetics, Genes, env, HIV-1/genetics, Humans, Intracellular Fluid/metabolism, Introns, Karyopherins/metabolism, Nuclear Proteins/metabolism, Plasmids, RNA Splicing, RNA, Messenger, RNA, Viral/metabolism, Receptors, Cytoplasmic and Nuclear, Ribonucleoproteins, Serine-Arginine Splicing Factors, Transfection, Virion, rev Gene Products, Human Immunodeficiency Virus, tat Gene Products, Human Immunodeficiency Virus
Pubmed
Web of science
Création de la notice
19/11/2007 13:19
Dernière modification de la notice
20/08/2019 14:01
Données d'usage