Regulation of replicative histone RNA metabolism by the histone chaperone ASF1.
Détails
ID Serval
serval:BIB_235759FC19DF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Regulation of replicative histone RNA metabolism by the histone chaperone ASF1.
Périodique
Molecular cell
ISSN
1097-4164 (Electronic)
ISSN-L
1097-2765
Statut éditorial
Publié
Date de publication
15/02/2024
Peer-reviewed
Oui
Volume
84
Numéro
4
Pages
791-801.e6
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
In S phase, duplicating and assembling the whole genome into chromatin requires upregulation of replicative histone gene expression. Here, we explored how histone chaperones control histone production in human cells to ensure a proper link with chromatin assembly. Depletion of the ASF1 chaperone specifically decreases the pool of replicative histones both at the protein and RNA levels. The decrease in their overall expression, revealed by total RNA sequencing (RNA-seq), contrasted with the increase in nascent/newly synthesized RNAs observed by 4sU-labeled RNA-seq. Further inspection of replicative histone RNAs showed a 3' end processing defect with an increase of pre-mRNAs/unprocessed transcripts likely targeted to degradation. Collectively, these data argue for a production defect of replicative histone RNAs in ASF1-depleted cells. We discuss how this regulation of replicative histone RNA metabolism by ASF1 as a "chaperone checkpoint" fine-tunes the histone dosage to avoid unbalanced situations deleterious for cell survival.
Mots-clé
Humans, Histones/genetics, Histones/metabolism, Histone Chaperones/genetics, Cell Cycle Proteins/genetics, Cell Cycle Proteins/metabolism, DNA Replication, Molecular Chaperones/genetics, Molecular Chaperones/metabolism, RNA/genetics, Saccharomyces cerevisiae Proteins/metabolism, 3′ end processing, ASF1, CAF-1, RNA metabolism, anti-silencing function 1, chromatin assembly factor-1, histone chaperone, replicative histone
Pubmed
Web of science
Création de la notice
13/05/2024 8:49
Dernière modification de la notice
14/05/2024 7:59