Maturation-dependent neurotoxicity of lead acetate in vitro: implication of glial reactions.

Détails

ID Serval
serval:BIB_23011
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Maturation-dependent neurotoxicity of lead acetate in vitro: implication of glial reactions.
Périodique
Journal of Neuroscience Research
Auteur⸱e⸱s
Zurich M.G., Eskes C., Honegger P., Bérode M., Monnet-Tschudi F.
ISSN
0360-4012[print], 0360-4012[linking]
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
70
Numéro
1
Pages
108-116
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Despite a wealth of data on the neurotoxic effects of lead at the cellular and molecular levels, the reasons for its development-dependent neurotoxicity are still unclear. Here, the maturation-dependent effects of lead acetate were analyzed in immature and differentiated brain cells cultured in aggregates. Markers of general cytotoxicity as well as cell-type-specific markers of glial and neuronal cells showed that immature brain cells were more sensitive to lead than the differentiated counterparts, demonstrating that the development-dependent neurotoxicity of lead can be reproduced in aggregating brain cell cultures. After 10 days of treatment, astrocytes were found to be more affected by lead acetate than neurons in immature cultures, and microglial cells were strongly activated. Eleven days after cessation of the treatment, lead acetate caused a partial loss of astrocytes and an intense reactivity of the remaining ones. Furthermore, microglial cells expressed a macrophagic phenotype, and the loss of activity of neuron-specific enzymes was aggravated. In differentiated cultures, no reactive gliosis was found. It is hypothetized that the intense glial reactions (microgliosis and astrogliosis) observed in immature cultures contribute to the development-dependent neurotoxicity of lead.
Mots-clé
Animals, Astrocytes/cytology, Astrocytes/drug effects, Carbon Isotopes, Cell Differentiation/drug effects, Cells, Cultured, Choline O-Acetyltransferase/metabolism, Fetus, Glial Fibrillary Acidic Protein/metabolism, Glutamate Decarboxylase/metabolism, Glutamate Synthase/metabolism, Immunohistochemistry, Lectins/chemistry, Neuroglia/cytology, Neuroglia/drug effects, Neurons/cytology, Neurons/drug effects, Organometallic Compounds/toxicity, Rats, Telencephalon, Thymidine/metabolism
Pubmed
Web of science
Création de la notice
19/11/2007 12:18
Dernière modification de la notice
20/08/2019 13:00
Données d'usage