Nano Secondary Ion Mass Spectrometry Imaging of Dopamine Distribution Across Nanometer Vesicles.

Détails

ID Serval
serval:BIB_23009CAEEF06
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Etude de cas (case report): rapporte une observation et la commente brièvement.
Collection
Publications
Institution
Titre
Nano Secondary Ion Mass Spectrometry Imaging of Dopamine Distribution Across Nanometer Vesicles.
Périodique
ACS nano
Auteur⸱e⸱s
Lovrić J., Dunevall J., Larsson A., Ren L., Andersson S., Meibom A., Malmberg P., Kurczy M.E., Ewing A.G.
ISSN
1936-0851
ISSN-L
1936-086X
Statut éditorial
Publié
Date de publication
2017
Peer-reviewed
Oui
Volume
11
Pages
3446-3455
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
We report an approach to spatially resolve the content across nanometer neuroendocrine vesicles in nerve-like cells by correlating super high-resolution mass spectrometry imaging, NanoSIMS, with transmission electron microscopy (TEM). Furthermore, intracellular electrochemical cytometry at nanotip electrodes is used to count the number of molecules in individual vesicles to compare to imaged amounts in vesicles. Correlation between the NanoSIMS and TEM provides nanometer resolution of the inner structure of these organelles. Moreover, correlation with electrochemical methods provides a means to quantify and relate vesicle neurotransmitter content and release, which is used to explain the slow transfer of dopamine between vesicular compartments. These nanoanalytical tools reveal that dopamine loading/unloading between vesicular compartments, dense core and halo solution, is a kinetically limited process. The combination of NanoSIMS and TEM has been used to show the distribution profile of newly synthesized dopamine across individual vesicles. Our findings suggest that the vesicle inner morphology might regulate the neurotransmitter release event during open and closed exocytosis from dense core vesicles with hours of equilibrium needed to move significant amounts of catecholamine from the protein dense core despite its nanometer size.
Mots-clé
NanoSIMS, electrochemistry, nanocompartments, nanoimaging, vesicle content
Pubmed
Web of science
Site de l'éditeur
Création de la notice
17/10/2018 12:05
Dernière modification de la notice
20/08/2019 14:00
Données d'usage