Intestinal bacteria condition dendritic cells to promote IgA production.

Détails

Ressource 1Télécharger: BIB_22B2BF0BE2BA.P001.pdf (403.78 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_22B2BF0BE2BA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Intestinal bacteria condition dendritic cells to promote IgA production.
Périodique
PLoS ONE
Auteur(s)
Massacand J.C., Kaiser P., Ernst B., Tardivel A., Bürki K., Schneider P., Harris N.L.
ISSN
1932-6203
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
3
Numéro
7
Pages
e2588
Langue
anglais
Résumé
Immunoglobulin (Ig) A represents the predominant antibody isotype produced at the intestinal mucosa, where it plays an important role in limiting the penetration of commensal intestinal bacteria and opportunistic pathogens. We show in mice that Peyer's Patch-derived dendritic cells (PP-DC) exhibit a specialized phenotype allowing the promotion of IgA production by B2 cells. This phenotype included increased expression of the retinaldehyde dehydrogenase 1 (RALDH1), inducible nitric oxide synthase (iNOS), B cell activating factor of the tumor necrosis family (BAFF), a proliferation-inducing ligand (APRIL), and receptors for the neuropeptide vasoactive intestinal peptide (VIP). The ability of PP-DC to promote anti-CD40 dependent IgA was partially dependent on retinoic acid (RA) and transforming growth factor (TGF)-beta, whilst BAFF and APRIL signaling were not required. Signals delivered by BAFF and APRIL were crucial for CD40 independent IgA production, although the contribution of B2 cells to this pathway was minimal. The unique ability of PP-DC to instruct naïve B cells to differentiate into IgA producing plasma cells was mainly imparted by the presence of intestinal commensal bacteria, and could be mimicked by the addition of LPS to the culture. These data indicate that exposure to pathogen-associated molecular patterns present on intestinal commensal bacteria condition DC to express a unique molecular footprint that in turn allows them to promote IgA production.
Mots-clé
Animals, Cell Differentiation, Dendritic Cells, Flow Cytometry, Immunoglobulin A, Intestine, Small, Mice, Mice, Inbred C57BL, Peyer's Patches, Phenotype
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/02/2009 20:00
Dernière modification de la notice
20/08/2019 13:00
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