Long-Term Engraftment of Primary Bone Marrow Stromal Cells Repairs Niche Damage and Improves Hematopoietic Stem Cell Transplantation.

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Etat: Public
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ID Serval
serval:BIB_22612957789F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Long-Term Engraftment of Primary Bone Marrow Stromal Cells Repairs Niche Damage and Improves Hematopoietic Stem Cell Transplantation.
Périodique
Cell stem cell
Auteur(s)
Abbuehl J.P., Tatarova Z., Held W., Huelsken J.
ISSN
1875-9777 (Electronic)
ISSN-L
1875-9777
Statut éditorial
Publié
Date de publication
03/08/2017
Peer-reviewed
Oui
Volume
21
Numéro
2
Pages
241-255.e6
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Hematopoietic stem cell (HSC) transplantation represents a curative treatment for various hematological disorders. However, delayed reconstitution of innate and adaptive immunity often causes fatal complications. HSC maintenance and lineage differentiation are supported by stromal niches, and we now find that bone marrow stroma cells (BMSCs) are severely and permanently damaged by the pre-conditioning irradiation required for efficient HSC transplantation. Using mouse models, we show that stromal insufficiency limits the number of donor-derived HSCs and B lymphopoiesis. Intra-bone transplantation of primary, but not cultured, BMSCs quantitatively reconstitutes stroma function in vivo, which is mediated by a multipotent NT5E(+) (CD73)(+) ENG(-) (CD105)(-) LY6A(+) (SCA1)(+) BMSC subpopulation. BMSC co-transplantation doubles the number of functional, donor-derived HSCs and significantly reduces clinically relevant side effects associated with HSC transplantation including neutropenia and humoral immunodeficiency. These data demonstrate the potential of stroma recovery to improve HSC transplantation.
Mots-clé
MSC, bone marrow transplantation, hematopoiesis, hematopoietic stem cells, skeletal stem cells
Pubmed
Web of science
Création de la notice
05/09/2017 16:38
Dernière modification de la notice
04/05/2021 5:37
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