In recent years, wearable epidermal sweat sensors have received extensive attention owing to their great potential to provide personalized information on the health status of individuals at the molecular level. For on-demand medical analysis of sweat in sedentary conditions, a cost-effective wearable integrated platform combining sweat stimulation, sampling, transport, and analysis is highly desirable. In this work, a printed iontophoretic system integrated into a microfluidic sensing platform, which combines sweat induction, collection, and real-time analysis of sweat-ions into a single patch for on-demand sweat monitoring on human subjects in stationary conditions is reported. The incorporation of microfluidics features facilitates sweat sampling, collection, and guiding through capillary effect. The multisensing sensor array exhibits sensitivity close to Nernstian behavior for sodium, potassium, and pH. The correlation between the concentrations of ions measured with the sweat patch and with ion chromatography analysis demonstrates the applicability of the system for real-time point-of-care monitoring of the health status of individuals. Furthermore, the sweat patch electronic interface with wireless transmission enables real-time data monitoring and storage over a cloud platform. This printed iontophoretic-integrated fluidic sweat patch provides a cost-effective solution for the on-demand analysis of sweat components for healthcare applications.