Distinction of virgin and memory T lymphocytes. Stable acquisition of the Pgp-1 glycoprotein concomitant with antigenic stimulation

Détails

ID Serval
serval:BIB_222AA841B96C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Distinction of virgin and memory T lymphocytes. Stable acquisition of the Pgp-1 glycoprotein concomitant with antigenic stimulation
Périodique
Journal of Immunology
Auteur(s)
Budd  R. C., Cerottini  J. C., Horvath  C., Bron  C., Pedrazzini  T., Howe  R. C., MacDonald  H. R.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
05/1987
Volume
138
Numéro
10
Pages
3120-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 15
Résumé
The Pgp-1 glycoprotein was identified on a minor (27%) subset of peripheral Lyt-2+ or L3T4+ T cells. In contrast, mature medullary-type thymocytes (Lyt-2+ L3T4-, Lyt-2- L3T4+) were nearly devoid of cells expressing detectable surface Pgp-1. The appearance of peripheral Pgp-1- T cells was found to be thymus dependent, as demonstrated by the diminished proportion of Pgp-1- T cells after thymectomy and their virtual absence in athymic nude mice. The subsequent acquisition of surface Pgp-1 was found to be a stable differentiation event occurring concomitantly with primary antigenic stimulation; selected Pgp-1- mature T cells from thymus or periphery acquired constitutive expression of Pgp-1 after stimulation in vitro with alloantigen or mitogens. These observations were extended by studies in vivo showing that immunization with various antigens augmented the percentage of Pgp-1+ spleen cells within the Lyt-2+ subset. Furthermore, the frequencies of antigen-specific CTLp, after immunization by any of three different antigens tested, were greatly enriched in the Pgp-1+ compared with the Pgp-1- subpopulations. Peritoneal exudate Lyt-2+ cells, after a localized allograft rejection, demonstrated a particularly prominent Pgp-1+ subpopulation (78%) that contained virtually all the allospecific cytolytic activity. A model consistent with all of these data proposes that mature thymocytes lacking surface Pgp-1 upon emigration to the periphery acquire its expression at the time of primary antigenic stimulation. Hence, expression of Pgp-1 among peripheral T cells is an important differentiation marker for identifying antigen-stimulated memory T cells.
Mots-clé
Animals Antigens, Differentiation, T-Lymphocyte Antigens, Ly/analysis Antigens, Surface/*analysis Cells, Cultured Female Glycoproteins/*analysis *Immunologic Memory Lymphocyte Activation Mice Mice, Inbred C57BL Mice, Inbred Strains Mice, Nude Receptors, Lymphocyte Homing T-Lymphocytes/analysis/*classification/immunology T-Lymphocytes, Cytotoxic/analysis Thymus Gland/cytology
Pubmed
Web of science
Création de la notice
28/01/2008 12:13
Dernière modification de la notice
20/08/2019 13:59
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