The C57BL/6J Mouse Exhibits Sporadic Congenital Portosystemic Shunts.

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_22298F21764D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The C57BL/6J Mouse Exhibits Sporadic Congenital Portosystemic Shunts.
Périodique
Plos One
Auteur(s)
Cudalbu C., McLin V.A., Lei H., Duarte J.M., Rougemont A.L., Oldani G., Terraz S., Toso C., Gruetter R.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
8
Numéro
7
Pages
e69782
Langue
anglais
Notes
Publication types: Journal Article
Résumé
C57BL/6 mice are the most widely used strain of laboratory mice. Using in vivo proton Magnetic Resonance Spectroscopy ((1)H MRS), we have repeatedly observed an abnormal neurochemical profile in the brains of both wild-type and genetically modified mice derived from the C57BL/6J strain, consisting of a several fold increase in cerebral glutamine and two fold decrease in myo-inositol. This strikingly abnormal neurochemical "phenotype" resembles that observed in chronic liver disease or portosystemic shunting and appeared to be independent of transgene, origin or chow and was not associated with liver failure. As many as 25% of animals displayed the abnormal neurochemical profile, questioning the reliability of this model for neurobiology. We conducted an independent study to determine if this neurochemical profile was associated with portosystemic shunting. Our results showed that 100% of the mice with high brain glutamine displayed portosystemic shunting by concomitant portal angiography while all mice with normal brain glutamine did not. Since portosystemic shunting is known to cause alterations in gene expression in many organs including the brain, we conclude that portosystemic shunting may be the most significant problem associated with C57BL/6J inbreeding both for its effect on the central nervous system and for its systemic repercussions.
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/08/2013 8:36
Dernière modification de la notice
20/08/2019 12:59
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