Skin permeation and metabolism of di(2-ethylhexyl) phthalate (DEHP).

Détails

Ressource 1Télécharger: 2014_Hopf_SkinToxLet_Postprint_pdg.pdf (440.41 [Ko])
Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_21FB6948A8F3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Skin permeation and metabolism of di(2-ethylhexyl) phthalate (DEHP).
Périodique
Toxicology letters
Auteur(s)
Hopf Nancy B., Berthet Aurélie, Vernez David, Langard Emilie, Spring Philipp, Gaudin René
ISSN
1879-3169 (Electronic)
ISSN-L
0378-4274
Statut éditorial
Publié
Date de publication
03/01/2014
Peer-reviewed
Oui
Volume
224
Numéro
1
Pages
47-53
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Phthalates are suspected to be endocrine disruptors. Di(2-ethylhexyl) phthalate (DEHP) is assumed to have low dermal absorption; however, previous in vitro skin permeation studies have shown large permeation differences. Our aims were to determine DEHP permeation parameters and assess extent of skin DEHP metabolism among workers highly exposed to these lipophilic, low volatile substances. Surgically removed skin from patients undergoing abdominoplasty was immediately dermatomed (800 μm) and mounted on flow-through diffusion cells (1.77 cm(2)) operating at 32°C with cell culture media (aqueous solution) as the reservoir liquid. The cells were dosed either with neat DEHP or emulsified in aqueous solution (166 μg/ml). Samples were analysed by HPLC-MS/MS. DEHP permeated human viable skin only as the metabolite MEHP (100%) after 8h of exposure. Human skin was able to further oxidize MEHP to 5-oxo-MEHP. Neat DEHP applied to the skin hardly permeated skin while the aqueous solution readily permeated skin measured in both cases as concentration of MEHP in the receptor liquid. DEHP pass through human skin, detected as MEHP only when emulsified in aqueous solution, and to a far lesser degree when applied neat to the skin. Using results from older in vitro skin permeation studies with non-viable skin may underestimate skin exposures. Our results are in overall agreement with newer phthalate skin permeation studies.

Mots-clé
Diethylhexyl Phthalate/metabolism, Humans, Permeability, Skin/metabolism, 117-81-7, 2cx-MMHP, 5OH-MEHP, 5cx-MEPP, 5oxo-MEHP, BSA, CV, DAD, DBP, DEHP, Di(2-ethylhexyl) phthalate, DiDP, DiNP, EFSA, EPA, ESI, EU, European Food Safety Authority, European Union, GHS, HGP, HMWP, HPLC, High-molecular-weight phthalates, Human, J, Kp, MBP, MEHP, OECD, Organisation for Economic Co-operation and Development, Percutaneous permeation, SC, Skin, TDI, TEWL, Tolerable Daily Intake, US Environmental Protection Agency, a high-performance liquid chromatograph, bovine serum albumin, coefficients of variation, dermally absorbed dose, di-butyl phthalate, diethyl hexyl phthalate, diisodecyl phthalate, diisononyl phthalate, electrospray interface, globally harmonized system of classification and labelling of chemicals, hairless guinea pig, lag time, mono(2-(carboxymethyl)hexyl) phthalate, mono-(2-ethyl-5-carboxypentyl) phthalate, mono-(2-ethyl-5-hydroxyhexyl) phthalate, mono-(2-ethyl-5-oxohexyl) phthalate, mono-(2-ethylhexyl) phthalate, mono-butyl phthalate, permeation coefficient, permeation rate, stratum corneum, trans epidermal water loss, τ
Pubmed
Web of science
Création de la notice
31/10/2013 16:46
Dernière modification de la notice
20/08/2019 13:58
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