Cell-Free Assay for Inflammasome Activation.

Détails

ID Serval
serval:BIB_2191EA9C70BB
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Cell-Free Assay for Inflammasome Activation.
Périodique
Methods in molecular biology
Auteur(s)
Jamilloux Y., Martinon F.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
1417
Pages
207-215
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Inflammasomes are multiprotein complexes, which assembly results in caspase-1 activation and subsequent IL-1β and IL-18 activation and secretion. In a cell-free system, based on cytosols of normally growing cells, the disruption of the cell membrane spontaneously activates the inflammasome. Studying the activation of the inflammasome in cytosolic extracts provides multiple advantages, as it is synchronized, rapid, strong, and mostly plasma membrane-free. This protocol covers the methods required to prepare cell lysates and study inflammasome activation using different read-outs. General considerations are provided that may help in the design of modified methods. This assay can be useful to study potential inflammasome interactors and the signaling pathways involved in its activation.

Mots-clé
Cell-Free System/metabolism, Cytosol/metabolism, Humans, Inflammasomes/metabolism, THP-1 Cells, ASC, Cell-free assay, IL-1β, Inflammasome, NLRP3
Pubmed
Création de la notice
26/05/2016 13:46
Dernière modification de la notice
20/08/2019 13:58
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