Spo0A regulates chromosome copy number during sporulation by directly binding to the origin of replication in Bacillus subtilis.

Détails

ID Serval
serval:BIB_216A59157F2D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Spo0A regulates chromosome copy number during sporulation by directly binding to the origin of replication in Bacillus subtilis.
Périodique
Molecular Microbiology
Auteur(s)
Boonstra M., de Jong I.G., Scholefield G., Murray H., Kuipers O.P., Veening J.W.
ISSN
1365-2958 (Electronic)
ISSN-L
0950-382X
Statut éditorial
Publié
Date de publication
2013
Volume
87
Numéro
4
Pages
925-938
Langue
anglais
Résumé
When starved, Bacillus subtilis cells can enter the developmental programme of endospore formation by activation of the master transcriptional regulator Spo0A. Correct chromosome copy number is crucial for the production of mature and fully resistant spores. The production and maintenance of one chromosome for the mother cell and one copy for the forespore requires accurate co-ordination between DNA replication and initiation of sporulation. Here, we show that Spo0A regulates chromosome copy number by directly binding to a number of Spo0A binding sites that are present near the origin of replication (oriC). We demonstrate that cells lacking three specific Spo0A binding sites at oriC display increased chromosome copy numbers when sporulation is induced. Our data support the hypothesis that Spo0A directly controls DNA replication during sporulation by binding to oriC.
Mots-clé
Bacillus subtilis/genetics, Bacillus subtilis/growth & development, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Chromosomes, Bacterial/genetics, Chromosomes, Bacterial/metabolism, Gene Dosage, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Developmental, Protein Binding, Replication Origin, Spores, Bacterial/genetics, Spores, Bacterial/growth & development, Transcription Factors/genetics, Transcription Factors/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/10/2016 15:31
Dernière modification de la notice
20/08/2019 12:58
Données d'usage