A 35.7 kb DNA fragment from the Bacillus subtilis chromosome containing a putative 12.3 kb operon involved in hexuronate catabolism and a perfectly symmetrical hypothetical catabolite-responsive element.

Détails

ID Serval
serval:BIB_2080693C9E72
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A 35.7 kb DNA fragment from the Bacillus subtilis chromosome containing a putative 12.3 kb operon involved in hexuronate catabolism and a perfectly symmetrical hypothetical catabolite-responsive element.
Périodique
Microbiology
Auteur(s)
Rivolta C., Soldo B., Lazarevic V., Joris B., Mauël C., Karamata D.
ISSN
1350-0872
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
144
Numéro
Pt 4
Pages
877-884
Langue
anglais
Résumé
The Bacillus subtilis strain 168 chromosomal region extending from 109 degrees to 112 degrees has been sequenced. Among the 35 ORFs identified, cotT and rapA were the only genes that had been previously mapped and sequenced. Out of ten ORFs belonging to a single putative transcription unit, seven are probably involved in hexuronate catabolism. Their sequences are homologous to Escherichia coli genes exuT, uidB, uxaA, uxaB, uxaC, uxuA and uxuB, which are all required for the uptake of free D-glucuronate, D-galacturonate and beta-glucuronide, and their transformation into glyceraldehyde 3-phosphate and pyruvate via 2-keto-3-deoxygluconate. The remaining three ORFs encode two dehydrogenases and a transcriptional regulator. The operon is preceded by a putative catabolite-responsive element (CRE), located between a hypothetical promoter and the RBS of the first gene. This element, the longest and the only so far described that is fully symmetrical, consists of a 26 bp palindrome matching the theoretical B. subtilis CRE sequence. The remaining predicted amino acid sequences that share homologies with other proteins comprise: a cytochrome P-450, a glycosyltransferase, an ATP-binding cassette transporter, a protein similar to the formate dehydrogenase alpha-subunit (FdhA), protein similar to NADH dehydrogenases, and three homologues of polypeptides that have undefined functions.
Mots-clé
Bacillus subtilis, Base Sequence, Chromosome Mapping, Chromosome Walking, Chromosomes, Bacterial, Cyclic AMP Receptor Protein, Genes, Bacterial, Hexuronic Acids, Molecular Sequence Data, Open Reading Frames, Operon, Polymerase Chain Reaction, Sequence Alignment
Pubmed
Web of science
Création de la notice
24/01/2008 14:14
Dernière modification de la notice
20/08/2019 12:56
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