Protein kinase C desensitization by phorbol esters and its impact on growth of human breast cancer cells

Détails

ID Serval
serval:BIB_2053365B172B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Protein kinase C desensitization by phorbol esters and its impact on growth of human breast cancer cells
Périodique
Biochemical and Biophysical Research Communications
Auteur(s)
Fabbro  D., Regazzi  R., Costa  S. D., Borner  C., Eppenberger  U.
ISSN
0006-291X (Print)
Statut éditorial
Publié
Date de publication
02/1986
Volume
135
Numéro
1
Pages
65-73
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb 26
Résumé
Active phorbol esters such as TPA (12-0-tetra-decanoylphorbol-13-acetate) inhibited growth of mammary carcinoma cells (MCF-7 greater than BT-20 greater than MDA-MB-231 greater than = ZR-75-1 greater than HBL-100) with the exception of T-47-D cells presumably by interacting with the phospholipid/Ca2+-dependent protein kinase (PKC). The nonresponsive T-47-D cells exhibited the lowest PKC activity. A rapid (30 min) TPA-dependent translocation of cytosolic PKC to membranes was found in the five TPA-sensitive cell without affecting cell growth. However, TPA-treatment of more than 10 hours inhibited reversibly the growth of TPA-responsive cells. This effect coincided with the complete loss of cellular PKC activity due to the proteolysis of the translocated membrane-bound PKC holoenzyme (75K) into 60K and 50K PKC fragments. Resumption of cell growth after TPA-removal was closely related to the specific reappearance of the PKC holoenzyme activity (75K) in the TPA-responsive human mammary tumor cell lines suggesting an involvement of PKC in growth regulation.
Mots-clé
Breast Neoplasms/*enzymology Cell Compartmentation Cell Division/drug effects Cell Line Cytosol/enzymology Female Growth Inhibitors Humans Membranes/enzymology Peptide Hydrolases/metabolism Phorbol Esters/*metabolism Protein Kinase C/*metabolism Protein Kinases/metabolism Time Factors
Pubmed
Web of science
Création de la notice
24/01/2008 14:30
Dernière modification de la notice
20/08/2019 12:56
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