Down-regulation of NOSII gene expression by iontophoresis of anti-sense oligonucleotide in endotoxin-induced uveitis.

Détails

ID Serval
serval:BIB_20250BBC0986
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Down-regulation of NOSII gene expression by iontophoresis of anti-sense oligonucleotide in endotoxin-induced uveitis.
Périodique
Biochemical and Biophysical Research Communications
Auteur(s)
Voigt M., de Kozak Y., Halhal M., Courtois Y., Behar-Cohen F.
ISSN
0006-291X (Print)
ISSN-L
0006-291X
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
295
Numéro
2
Pages
336-341
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Transcorneoscleral iontophoresis was used to enhance ocular penetration of a 21-bp NH(2) protected anti-NOSII oligonucleotides (ODNs) (fluorescein or infrared-41 labeled) in Lewis rats. Both histochemical localization and acrylamide sequencing gels were used. To evaluate the potential to down-regulate NOSII expression in the rat model of endotoxin-induced uveitis (EIU), anti-sense NOSII ODN, scrambled ODN or saline were iontophorezed into these animals' eyes. Iontophoresis facilitated the penetration of intact ODNs into the intraocular tissues of the rat eye and only the eyes receiving ODNs and electrical current demonstrated intact ODNs within the ocular tissues of both segments of the eye. Iontophoresis of anti-NOSII ODN significantly down-regulated the expression of NOSII expression in iris/ciliary body compared to the saline or scrambled ODN treated eyes. Nitrite production was also significantly reduced in the anti-NOSII applied eyes compared to those treated with saline. Using this system, intraocular delivery of ODNs can be significantly enhanced increasing the potential for successful gene therapy for human eye diseases.
Mots-clé
Animals, Base Sequence, DNA Primers, Down-Regulation/drug effects, Endotoxins/toxicity, Iontophoresis, Nitric Oxide Synthase/genetics, Nitric Oxide Synthase Type II, Oligonucleotides, Antisense/pharmacology, Rats, Rats, Inbred Lew, Uveitis/chemically induced, Uveitis/enzymology
Pubmed
Web of science
Création de la notice
08/04/2014 11:59
Dernière modification de la notice
20/08/2019 13:56
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