Cell-free reconstitution of microautophagy in yeast.

Détails

ID Serval
serval:BIB_1FBFE24AFD60
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cell-free reconstitution of microautophagy in yeast.
Périodique
Methods in Enzymology
Auteur⸱e⸱s
Mayer A.
ISSN
1557-7988 (Electronic)
ISSN-L
0076-6879
Statut éditorial
Publié
Date de publication
2008
Volume
451
Pages
151-162
Langue
anglais
Résumé
Microautophagy is the direct uptake of soluble or particulate cellular constituents into lysosomes. Here, I describe methods to reconstitute and study this process in vitro, using vacuoles (lysosomes) from the yeast Saccharomyces cerevisiae as model organelles. Protocols to grow the cells, isolate vacuoles from them, and to induce microautophagy of soluble tracers are presented.
Mots-clé
Autophagy/physiology, Cell-Free System/metabolism, Cytoplasm/metabolism, Saccharomyces cerevisiae/cytology, Saccharomyces cerevisiae/metabolism, Vacuoles/metabolism
Pubmed
Web of science
Création de la notice
24/03/2009 15:13
Dernière modification de la notice
20/08/2019 12:55
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