DIP-STR: A new marker for resolving unbalanced DNA mixtures

Détails

ID Serval
serval:BIB_1F360559B904
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
DIP-STR: A new marker for resolving unbalanced DNA mixtures
Périodique
Forensic Science International: Genetics Supplement Series
Auteur(s)
Hall D., Castella V.
ISSN
1875-1768
ISSN-L
1875-1768
Statut éditorial
Publié
Date de publication
2011
Volume
3
Pages
e1-e2
Langue
anglais
Résumé
The genetic characterization of unbalanced mixed stains remains an important area where improvementis imperative. Most cases of aggression, homicide and sexual assault produce biological traces withrelatively large amount of the victim's DNA and small amount of the aggressor's DNA. If this ratio issmaller than 1:10 it is currently not possible to obtain a conventional autosomal DNA profile of the minorcontributor, with potential loss of crucial DNA evidence. Y-STR analysis represents a solution for somecases but has several limitations. We propose here a method based on a new compound genetic markerformed by a Deletion/Insertion Polymorphism (DIP) linked to a Short Tandem Repeat polymorphism(STR), that we name DIP-STR. By means of allele-specific amplifications of DIP-STR haplotypes, we canproduce a high resolution autosomal DNA profile of a donor that contributes less than 0.1% to a DNAmixture. Based on these features DIP-STR markers may outperform conventional Y-STR markers inmixed stain analysis.
Mots-clé
DNA mixture analysis, insertion/deletion polymorphism, sexual assault, allele-specific PCR
Création de la notice
28/02/2012 13:54
Dernière modification de la notice
20/08/2019 12:55
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