Differential expression of the ccn3 (nov) proto-oncogene in human prostate cell lines and tissues

Détails

ID Serval
serval:BIB_1E6B156B4D45
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Differential expression of the ccn3 (nov) proto-oncogene in human prostate cell lines and tissues
Périodique
Molecular Pathology
Auteur(s)
Maillard  M., Cadot  B., Ball  R. Y., Sethia  K., Edwards  D. R., Perbal  B., Tatoud  R.
ISSN
1366-8714 (Print)
Statut éditorial
Publié
Date de publication
08/2001
Volume
54
Numéro
4
Pages
275-80
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Résumé
AIMS: To investigate the expression of the human ccn3 (hccn3; nov) proto-oncogene, a member of the CCN family of proteins, in prostate epithelial cells and prostate tissue samples. METHODS: Normal adult prostate luminal epithelial cells immortalised by SV40 large T (PNT1A and PNT1B), metastatic tumours (LNCaP, DU-145, and PC-3), and prostate tissue samples from patients with benign prostatic hyperplasia (BPH) and prostatic adenocarcinoma were used. hccn3 (nov) mRNA was measured by the reverse transcription polymerase chain reaction (RT-PCR) and hCCN3 (NOV) protein expression was determined by immunochemistry. RESULTS: hccn3 (nov) RNA values were higher in PC-3 cells than in the other prostate cell lines. The immortalised normal cell lines either did not express hccn3 (nov) RNA (PNT1B) or expressed very low amounts (PNT1A). BPH samples expressed variable amounts of hccn3 (nov) RNA. With the use of immunocytochemistry, all cell lines except PNT1A and PNT1B were shown to contain hCCN3 (NOV) protein. hCCN3 (NOV) was localised mainly in the epithelial compartment of BPH and adenocarcinoma samples, and there was evidence of luminal secretion. CONCLUSION: The overexpression of hccn3 (nov) RNA in cancer cell lines compared with other cell lines and its epithelial localisation in human prostate samples are consistent with a role for hCCN3 (NOV) in prostatic tumorigenesis.
Mots-clé
Adenocarcinoma/*genetics Adult Aged Aged, 80 and over Humans *Immediate-Early Proteins Immunohistochemistry *Intercellular Signaling Peptides and Proteins Male Middle Aged Oncogene Proteins, Viral/*genetics Prostate/chemistry Prostatic Hyperplasia/metabolism Prostatic Neoplasms/*genetics Proto-Oncogene Proteins/*genetics RNA/analysis Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
25/01/2008 13:59
Dernière modification de la notice
20/08/2019 13:54
Données d'usage