Quantitation of vitellogenin messenger RNA in the liver of male Xenopus toads during primary and secondary stimulation by estrogen.

Détails

ID Serval
serval:BIB_1E57F1337E82
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Quantitation of vitellogenin messenger RNA in the liver of male Xenopus toads during primary and secondary stimulation by estrogen.
Périodique
Cell
Auteur⸱e⸱s
Ryffel G.U., Wahli W., Weber R.
ISSN
0092-8674[print], 0092-8674[linking]
Statut éditorial
Publié
Date de publication
05/1977
Volume
11
Numéro
1
Pages
213-221
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
From livers of estrogen-stimulated female Xenopus toads, large quantities of estrogen-induced, poly(A)-containing RNA could be isolated, showing the same characteristics as vitellogenin mRNA obtained from hormone-treated males.
Using cDNA hybridization, vitellogenin mRNA was monitored in the cytoplasmic poly(A)-containing RNA of the liver of male toads during 13 days of primary and the initial phase of secondary stimulation with estrogen.
During primary stimulation, low amounts of vitellogenin mRNA, not exceeding 0.18% of the cytoplasmic poly(A)-containing RNA, were first detected after 12 hr of hormone treatment, and vitellogenin mRNA was found to increase on the average to 34% of the cytoplasmic poly(A)-containing RNA on the seventh day of hormone treatment. After 3 days of primary stimulation, accumulation of vitellogenin mRNA leveled off, showing no significant increase in the cytoplasm up to 13 days of hormone treatment. As judged from incorporation of 32PO4 into blood plasma proteins of males during primary stimulation, vitellogenin was first detected after 1 day, and its synthesis was found to increase dramatically until the thirteenth day of hormone treatment. This implies that there is a coincidence between appearance and extent of synthesis of vitellogenin and the abundance of vitellogenin mRNA in the cytoplasm, but there is evidence that during later phase of primary stimulation (day 3-13), the increase in synthesis of vitellogenin cannot be attributed anymore to a significant accumulation of vitellogenin mRNA.
In male Xenopus, estrogen-induced synthesis of vitellogenin is no more detectable 41 days after hormone injection, and the concentration of vitellogenin mRNA was found to be <0.03% of the cytoplasmic poly(A)-containing RNA. Secondary stimulation by estrogen of these animals results in an at least 30 fold faster accumulation of vitellogenin mRNA in the cytoplasm within the initial 12 hr of hormone treatment. This may explain the faster appearance of vitellogenin in the blood plasma.
Mots-clé
Animals, Cell-Free System, Cytoplasm/metabolism, DNA, Circular/biosynthesis, Estradiol/pharmacology, Female, Lipoproteins/biosynthesis, Liver/drug effects, Liver/metabolism, Male, Nucleic Acid Hybridization, Poly A/metabolism, RNA/metabolism, RNA, Messenger/biosynthesis, Vitellogenins/biosynthesis, Xenopus
Pubmed
Web of science
Création de la notice
24/01/2008 16:05
Dernière modification de la notice
20/08/2019 12:54
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