The oncogene phosphatidylinositol 3'-kinase catalytic subunit alpha promotes angiogenesis via vascular endothelial growth factor in ovarian carcinoma.

Détails

ID Serval
serval:BIB_1E34A35AFD0A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The oncogene phosphatidylinositol 3'-kinase catalytic subunit alpha promotes angiogenesis via vascular endothelial growth factor in ovarian carcinoma.
Périodique
Cancer Research
Auteur⸱e⸱s
Zhang L., Yang N., Katsaros D., Huang W., Park J.W., Fracchioli S., Vezzani C., Rigault de la Longrais I.A., Yao W., Rubin S.C., Coukos G.
ISSN
0008-5472 (Print)
ISSN-L
0008-5472
Statut éditorial
Publié
Date de publication
2003
Volume
63
Numéro
14
Pages
4225-4231
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
The gene of phosphatidylinositol 3-kinase catalytic subunit alpha (PIK3CA) has been implicated as an oncogene in ovarian cancer [L. Shayesteh et al., Nat. Genet., 21: 99-102, 1999]. In this study, we examined the expression of PIK3CA mRNA and its p110alpha protein product in human ovarian carcinoma and investigated its role in regulating angiogenesis via vascular endothelial growth factor (VEGF). PIK3CA mRNA was detected in 66.6% of stage I and 93.9% of advanced stage ovarian cancer specimens and in all 17 ovarian cancer cell lines. PIK3CA mRNA levels were significantly higher in invasive carcinomas compared with benign and low malignant potential neoplasms (P = 0.007), but no significant difference was seen between early and advanced stage carcinomas (P = 0.812). Strong expression of immunoreactive p110alpha was detected in tumor cells and/or stroma endothelium. PIK3CA expression in vivo positively correlated, both at the mRNA and the protein level, with the expression of VEGF as well as with the extent of microvascular development. Furthermore, PIK3CA mRNA overexpression positively correlated with increased proliferation and decreased apoptosis of tumor cells in vivo. In vitro, PIK3CA expression positively correlated with the expression of VEGF in ovarian cancer cells, whereas the phosphatidylinositol 3'-kinase inhibitor Ly294002 reduced both the constitutive and inducible expression of hypoxia-inducible factor-1alpha at the mRNA and protein levels and abrogated VEGF up-regulation by glucose starvation. Furthermore, Ly294002 suppressed cell proliferation and, at higher doses, induced marked apoptosis in ovarian cancer cells. Collectively, these data strongly indicate that PIK3CA supports ovarian cancer growth through multiple and independent pathways affecting cell proliferation, apoptosis and angiogenesis, and plays an important role in ovarian cancer progression.
Mots-clé
Apoptosis/physiology, Catalytic Domain, Cell Division/physiology, Chromones/pharmacology, Endothelial Growth Factors/biosynthesis, Endothelial Growth Factors/genetics, Enzyme Inhibitors/pharmacology, Female, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Humans, Hypoxia-Inducible Factor 1, alpha Subunit, Intercellular Signaling Peptides and Proteins/biosynthesis, Intercellular Signaling Peptides and Proteins/genetics, Lymphokines/biosynthesis, Lymphokines/genetics, Morpholines/pharmacology, Neovascularization, Pathologic/enzymology, Neovascularization, Pathologic/genetics, Ovarian Neoplasms/blood supply, Ovarian Neoplasms/enzymology, Phosphatidylinositol 3-Kinases/antagonists & inhibitors, Phosphatidylinositol 3-Kinases/biosynthesis, RNA, Messenger/biosynthesis, RNA, Messenger/genetics, Transcription Factors/metabolism, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factors
Pubmed
Web of science
Création de la notice
14/10/2014 11:43
Dernière modification de la notice
20/08/2019 12:54
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