Relation between liver progenitor cell expansion and extracellular matrix deposition in a CDE-induced murine model of chronic liver injury.

Détails

ID Serval
serval:BIB_1E0F63A07837
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Relation between liver progenitor cell expansion and extracellular matrix deposition in a CDE-induced murine model of chronic liver injury.
Périodique
Hepatology
Auteur⸱e⸱s
Van Hul N.K., Abarca-Quinones J., Sempoux C., Horsmans Y., Leclercq I.A.
ISSN
1527-3350 (Electronic)
ISSN-L
0270-9139
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
49
Numéro
5
Pages
1625-1635
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
further differentiation. This process is associated with extracellular matrix (ECM) remodeling. We analyzed LPC expansion and matrix accumulation in a choline-deficient, ethionine-supplemented (CDE) model of LPC proliferation. After day 3, CDE induced collagen deposits in the periportal ar a. Expansion of LPCs as assessed by increased number of cytokeratin 19 (CK19)-positive cells was first observed at day 7, while ECM accumulated 10 times more than in controls. Thereafter, LPCs and ECM increased in parallel. Furthermore, ECM not only accumulates prior to the increase in number of LPCs, but is also found in front of LPCs along the porto-venous gradient of lobular invasion. Double immunostaining revealed that LPCs are embedded in ECM at all times. Moreover, LPCs infiltrating the liver parenchyma are chaperoned by alpha-smooth muscle actin (alpha-SMA)-positive cells. Gene expression analyses confirmed these observations. The expression of CK19, alpha-fetoprotein, E-cadherin, and CD49f messenger RNA (mRNA), largely overexpressed by LPCs, significantly increased between day 7 and day 10. By contrast, at day 3 there was a rapid burst in the expression of components of the ECM, collagen I and laminin, as well as in alpha-SMA and connective tissue growth factor expression.
CONCLUSION: Our data demonstrate that, in a CDE model, ECM deposition and activation of matrix-producing cells occurred as an initial phase, prior to LPC expansion, and in front of LPCs along the porto-venous gradient of lobular invasion. Those observations may reveal a fundamental role for the established hepatic microenvironment or niche during the process of activation and differentiation of liver progenitor cells.
Mots-clé
Animals, Cell Differentiation, Cell Proliferation, Choline Deficiency/complications, Disease Models, Animal, Ethionine/toxicity, Extracellular Matrix/metabolism, Gene Expression Profiling, Hepatic Stellate Cells/physiology, Liver/cytology, Liver/metabolism, Liver Diseases/etiology, Liver Regeneration, Male, Mice, Mice, Inbred C57BL, Time Factors
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/01/2015 11:55
Dernière modification de la notice
20/08/2019 12:54
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