In vitro effect of corneal collagen cross-linking on corneal hydration properties and stiffness.

Détails

ID Serval
serval:BIB_1D764533A37D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vitro effect of corneal collagen cross-linking on corneal hydration properties and stiffness.
Périodique
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
Auteur(s)
Kontadakis G.A., Ginis H., Karyotakis N., Pennos A., Pentari I., Kymionis G.D., Pallikaris I.G.
ISSN
1435-702X (Electronic)
ISSN-L
0721-832X
Statut éditorial
Publié
Date de publication
02/2013
Peer-reviewed
Oui
Volume
251
Numéro
2
Pages
543-547
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The purpose of this study is to evaluate in-vitro the immediate effect of corneal collagen cross-linking (CXL) on corneal hydration and stiffness.
Forty-two corneal buttons from freshly enucleated porcine eyes were immersed in riboflavin 0.1% in dextran 20% dilution for 3 h in order for their hydration to reach equilibrium. Corneal buttons where divided into two groups; the first group was stored in dark conditions while the other group was irradiated with UV radiation (370 nm) for 30 min to simulate CXL according to the clinically applied protocol. After irradiation, all corneas were immersed in dextran 20% solution for 3 additional hours. Subsequently, each button underwent weighing, thickness measurement, and was mounted in a special device for the measurement of force versus deformation by compression. Finally, all corneal buttons were dehydrated for 48 h in a desiccating oven set at 62 °C and weighed again to obtain their dry mass. Hydration (%) of each button was calculated.
Mean corneal hydration in the irradiated and the non-irradiated group of corneas was 69.8 and 72.2%, respectively (p < 0.001). Differences in thickness and compressibility were not statistically significant. Thickness and hydration were positively correlated (Pearson's r = 0.714, p < 0.001).
CXL causes corneal dehydration that can be detected immediately after the procedure. This phenomenon may contribute to increased mechanical stiffness of the cornea. A change in stiffness by means of compressibility could not be detected in porcine corneas.
Mots-clé
Animals, Body Water/physiology, Collagen/metabolism, Cornea/physiology, Corneal Pachymetry, Corneal Stroma/metabolism, Cross-Linking Reagents/pharmacology, Elasticity/physiology, Photosensitizing Agents/pharmacology, Pliability/physiology, Riboflavin/pharmacology, Swine, Ultraviolet Rays, Water-Electrolyte Balance
Pubmed
Web of science
Création de la notice
01/10/2019 13:19
Dernière modification de la notice
06/10/2019 5:26
Données d'usage