Identification and characterization of a mammalian protein interacting with 20S proteasome precursors

Détails

ID Serval
serval:BIB_1CDCCA438D90
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification and characterization of a mammalian protein interacting with 20S proteasome precursors
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
Burri  L., Hockendorff  J., Boehm  U., Klamp  T., Dohmen  R. J., Levy  F.
ISSN
0027-8424 (Print)
Statut éditorial
Publié
Date de publication
09/2000
Volume
97
Numéro
19
Pages
10348-53
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 12
Résumé
The assembly of individual mammalian proteasome subunits into catalytically active 20S proteasome is not well understood. Herein, we report the identification and characterization of human and mouse homologues of the yeast proteasome maturating factor Ump1p. We delineate the region of hUMP1 implicated in the specific interaction with proteasome precursors and show that hUMP1 protein is absent from the mature form of the 20S proteasome. We also show that the transcript level of mammalian UMP1 is increased after IFN-gamma treatment and that mammalian UMP1 is functionally related to but not interchangeable with its yeast homologue.
Mots-clé
Amino Acid Sequence Animals Blotting, Northern Cloning, Molecular Cysteine Endopeptidases/*metabolism Humans Mice Mice, Inbred C57BL Molecular Chaperones/chemistry/genetics/*metabolism Molecular Sequence Data Multienzyme Complexes/*metabolism Proteasome Endopeptidase Complex Protein Binding RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Sequence Homology, Amino Acid
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:17
Dernière modification de la notice
20/08/2019 13:53
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