Cloning of a bovine renal epithelial Na+ channel subunit
Détails
ID Serval
serval:BIB_1C49AC26EA42
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cloning of a bovine renal epithelial Na+ channel subunit
Périodique
American Journal of Physiology
ISSN
0363-6143
Statut éditorial
Publié
Date de publication
09/1995
Volume
269
Numéro
3 Pt 1
Pages
C641-54
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep
Résumé
A bovine homologue of the rat and human epithelial Na+ channel subunits, alpha-rENaC and alpha-hENaC, was cloned. The cDNA clone, termed alpha-bENaC, was isolated from a bovine renal papillary collecting duct cDNA expression library. The bovine cDNA is 3,584 base pairs (bp) long, has an open reading frame of 2,094 bp encoding a 697-amino acid protein, and is 75-85% homologous to its rat and human counterparts. In vitro translation of the transcribed cRNA yields an 80-kDa polypeptide and one at 92 kDa in the presence of pancreatic microsomes. The clone exhibits consensus sequences for N-linked glycosylation and for phosphorylation by protein kinase C, but not for protein kinase A. After expression in Xenopus laevis oocytes, a small amiloride-sensitive Na+ conductance that exhibited inward rectification and a reversal potential greater than +30 mV, consistent with the predicted equilibrium potential for Na+, was identified. The expressed alpha-bENaC-associated Na+ current was not responsive to elevations in adenosine 3',5'-cyclic monophosphate but could be stimulated by phorbol 12-myristate 13-acetate, an activator of protein kinase C. alpha-bENaC also formed amiloride-sensitive chimeric channels when coexpressed with the rat beta- and gamma-ENaC subunits in Xenopus oocytes. alpha-bENaC therefore represents a novel isoform of a growing family of epithelial Na+ channels.
Mots-clé
Amino Acid Sequence
Animals
Antisense Elements (Genetics)/genetics
Base Sequence
Blotting, Northern
Cattle
*Cloning, Molecular
Epithelium/metabolism
Humans
Immunoblotting
Isomerism
Kidney/*metabolism
Molecular Probes/genetics
Molecular Sequence Data
Oocytes/metabolism
Protein Biosynthesis
Rats
Sequence Homology, Amino Acid
Sodium Channels/chemistry/*genetics/*metabolism
Xenopus laevis
Pubmed
Web of science
Création de la notice
24/01/2008 13:00
Dernière modification de la notice
20/08/2019 12:52