p107 regulates neural precursor cells in the mammalian brain.

Détails

ID Serval
serval:BIB_1BC92C013B56
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
p107 regulates neural precursor cells in the mammalian brain.
Périodique
The Journal of cell biology
Auteur⸱e⸱s
Vanderluit J.L., Ferguson K.L., Nikoletopoulou V., Parker M., Ruzhynsky V., Alexson T., McNamara S.M., Park D.S., Rudnicki M., Slack R.S.
ISSN
0021-9525 (Print)
ISSN-L
0021-9525
Statut éditorial
Publié
Date de publication
13/09/2004
Peer-reviewed
Oui
Volume
166
Numéro
6
Pages
853-863
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Here we show a novel function for Retinoblastoma family member, p107 in controlling stem cell expansion in the mammalian brain. Adult p107-null mice had elevated numbers of proliferating progenitor cells in their lateral ventricles. In vitro neurosphere assays revealed striking increases in the number of neurosphere forming cells from p107(-/-) brains that exhibited enhanced capacity for self-renewal. An expanded stem cell population in p107-deficient mice was shown in vivo by (a) increased numbers of slowly cycling cells in the lateral ventricles; and (b) accelerated rates of neural precursor repopulation after progenitor ablation. Notch1 was up-regulated in p107(-/-) neurospheres in vitro and brains in vivo. Chromatin immunoprecipitation and p107 overexpression suggest that p107 may modulate the Notch1 pathway. These results demonstrate a novel function for p107 that is distinct from Rb, which is to negatively regulate the number of neural stem cells in the developing and adult brain.
Mots-clé
Adenoviridae/genetics, Animals, Apoptosis, Blotting, Western, Brain/cytology, Bromodeoxyuridine/metabolism, Cell Division, Cells, Cultured, Gene Expression Regulation, Developmental, Immunohistochemistry, Membrane Proteins/metabolism, Mice, Mice, Knockout, Neurons/metabolism, Olfactory Bulb/cytology, RNA, Messenger/metabolism, Receptors, Notch, Retinoblastoma Protein/genetics, Stem Cells/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
04/06/2024 10:52
Dernière modification de la notice
05/06/2024 9:04
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